7f1j: Difference between revisions

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-'''Unreleased structure'''
-The entry 7f1j is ON HOLD  until Paper Publication
+==Designed enzyme RA61 M48K/I72D mutant: form III==
+<StructureSection load='7f1j' size='340' side='right'caption='[[7f1j]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7f1j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1J FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1j OCA], [https://pdbe.org/7f1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1j RCSB], [https://www.ebi.ac.uk/pdbsum/7f1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1j ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Natural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold.
-Authors: Fujioka, T., Oka, M., Numoto, N., Ito, N., Oda, M., Tanaka, F.
+Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions.,Fujioka T, Numoto N, Akama H, Shilpa K, Oka M, Roy PK, Krishna Y, Ito N, Baker D, Oda M, Tanaka F Chembiochem. 2021 Oct 26. doi: 10.1002/cbic.202100435. PMID:34698422<ref>PMID:34698422</ref>
-Description: Designed enzyme RA61 M48K/I72D mutant: form III
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Tanaka, F]]
+<div class="pdbe-citations 7f1j" style="background-color:#fffaf0;"></div>
-[[Category: Oka, M]]
-[[Category: Numoto, N]]
+==See Also==
-[[Category: Oda, M]]
+*[[Aldolase 3D structures|Aldolase 3D structures]]
-[[Category: Ito, N]]
+== References ==
-[[Category: Fujioka, T]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Fujioka T]]
+[[Category: Ito N]]
+[[Category: Numoto N]]
+[[Category: Oda M]]
+[[Category: Oka M]]
+[[Category: Tanaka F]]