Proteopedia

2n01: Difference between revisions

← Older edit
No edit summary
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==NMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SS==
==NMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SS==
<StructureSection load='2n01' size='340' side='right'caption='[[2n01]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2n01' size='340' side='right'caption='[[2n01]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2n01]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanac Xanac]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N01 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2n01]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N01 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2l4w|2l4w]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">virB9, XAC2620, XAC2622 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n01 OCA], [https://pdbe.org/2n01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n01 RCSB], [https://www.ebi.ac.uk/pdbsum/2n01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n01 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n01 OCA], [https://pdbe.org/2n01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n01 RCSB], [https://www.ebi.ac.uk/pdbsum/2n01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n01 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8PJB3_XANAC Q8PJB3_XANAC]
The type IV secretion system (T4SS) from the phytopathogen Xanthomonas citri (Xac) is a bactericidal nanomachine. The T4SS core complex is a ring composed of multiple copies of VirB7-VirB9-VirB10 subunits. Xac-VirB7 contains a disordered N-terminal tail (VirB7NT) that recognizes VirB9, and a C-terminal domain (VirB7CT) involved in VirB7 self-association. Here, we show that VirB7NT forms a short beta strand upon binding to VirB9 and stabilizes it. A tight interaction between them is essential for T4SS assembly and antibacterial activity. Abolishing VirB7 self-association or deletion of the VirB7 C-terminal domain impairs this antibacterial activity without disturbing T4SS assembly. These findings reveal protein interactions within the core complex that are critical for the stability and activity of a T4SS.
 
VirB7 and VirB9 Interactions Are Required for the Assembly and Antibacterial Activity of a Type IV Secretion System.,Oliveira LC, Souza DP, Oka GU, Lima FD, Oliveira RJ, Favaro DC, Wienk H, Boelens R, Farah CS, Salinas RK Structure. 2016 Oct 4;24(10):1707-1718. doi: 10.1016/j.str.2016.07.015. Epub 2016, Sep 1. PMID:27594685<ref>PMID:27594685</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2n01" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Xanac]]
[[Category: Xanthomonas citri pv. citri str. 306]]
[[Category: Boelens, R]]
[[Category: Boelens R]]
[[Category: Farah, S C]]
[[Category: Farah SC]]
[[Category: Oliveira, L C]]
[[Category: Oliveira LC]]
[[Category: Salinas, R K]]
[[Category: Salinas RK]]
[[Category: Souza, D P]]
[[Category: Souza DP]]
[[Category: Wienk, H]]
[[Category: Wienk H]]
[[Category: Lipoprotein]]
[[Category: Protein transport-protein transport complex]]
[[Category: Protein-peptide complex]]
[[Category: T4ss]]
[[Category: Virb7]]
[[Category: Virb9]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/w/2n01"