1eik: Difference between revisions

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-[[Image:1eik.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_1eik", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
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-{{STRUCTURE_1eik|  PDB=1eik  |  SCENE=  }}
-'''SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''
+==Solution Structure of RNA Polymerase Subunit RPB5 from Methanobacterium Thermoautotrophicum==
+<StructureSection load='1eik' size='340' side='right'caption='[[1eik]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1eik]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIK FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eik OCA], [https://pdbe.org/1eik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eik RCSB], [https://www.ebi.ac.uk/pdbsum/1eik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eik ProSAT], [https://www.topsan.org/Proteins/NESGC/1eik TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPO5_METTH RPO5_METTH] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00025]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eik_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eik ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. It is a proposed target for transcription activator proteins in eukaryotes, but the mechanism of interaction is not known. We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum. MtRBP5 contains a four-stranded beta-sheet platform supporting two alpha-helices, one on each side of the beta-sheet, resulting in an overall mushroom shape that does not appear to have any structural homologues in the structural database. The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein.
-==Overview==
+Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum.,Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6311-5. PMID:10841538<ref>PMID:10841538</ref>
-RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. It is a proposed target for transcription activator proteins in eukaryotes, but the mechanism of interaction is not known. We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum. MtRBP5 contains a four-stranded beta-sheet platform supporting two alpha-helices, one on each side of the beta-sheet, resulting in an overall mushroom shape that does not appear to have any structural homologues in the structural database. The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-EIK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIK OCA].
+</div>
+<div class="pdbe-citations 1eik" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum., Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6311-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10841538 10841538]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-[[Category: DNA-directed RNA polymerase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Single protein]]
+[[Category: Arrowsmith CH]]
-[[Category: Arrowsmith, C H.]]
+[[Category: Booth V]]
-[[Category: Booth, V.]]
+[[Category: Dharamsi A]]
-[[Category: Dharamsi, A.]]
+[[Category: Edwards AM]]
-[[Category: Edwards, A M.]]
+[[Category: Engel A]]
-[[Category: Engel, A.]]
+[[Category: Yee A]]
-[[Category: NESG, Northeast Structural Genomics Consortium.]]
-[[Category: Yee, A.]]
-[[Category: Nesg]]
-[[Category: Northeast structural genomics consortium]]
-[[Category: Ocsp]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Rna polymerase subunit]]
-[[Category: Rpb5]]
-[[Category: Rpbh]]
-[[Category: Structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:08:46 2008''