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==== | ==EV-D68 in complex with 8F12 Fab== | ||
<StructureSection load='7ec5' size='340' side='right'caption='[[7ec5]]' scene=''> | <StructureSection load='7ec5' size='340' side='right'caption='[[7ec5]], [[Resolution|resolution]] 2.89Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ec5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Enterovirus_D68 Enterovirus D68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EC5 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ec5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ec5 OCA], [https://pdbe.org/7ec5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ec5 RCSB], [https://www.ebi.ac.uk/pdbsum/7ec5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ec5 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.89Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ec5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ec5 OCA], [https://pdbe.org/7ec5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ec5 RCSB], [https://www.ebi.ac.uk/pdbsum/7ec5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ec5 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Enterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP and virion and in neutralizing different EV-D68 strains. A combination of 2H12 and 8F12 exhibits balanced and potent neutralization effects and confers broader protection in mice than single MAbs when given at onset of symptoms. Cryo-EM structures of EV-D68 virion complexed with 2H12 or 8F12 show that both antibodies bind to the canyon region of the virion, creating steric hindrance for sialic acid receptor binding. Additionally, 2H12 binding can impair virion integrity and trigger premature viral uncoating. We also capture an uncoating intermediate induced by 2H12 binding, not previously described for picornaviruses. Our study elucidates the structural basis and neutralizing mechanisms of the 2H12 and 8F12 MAbs and supports further development of the 2H12/8F12 cocktail as a broad-spectrum therapeutic agent against EV-D68 infections in humans. | |||
Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections.,Zhang C, Xu C, Dai W, Wang Y, Liu Z, Zhang X, Wang X, Wang H, Gong S, Cong Y, Huang Z Nat Commun. 2021 May 18;12(1):2904. doi: 10.1038/s41467-021-23199-5. PMID:34006855<ref>PMID:34006855</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ec5" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Enterovirus D68]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: Cong Y]] | |||
[[Category: Xu C]] | |||