7eg7: Difference between revisions

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-'''Unreleased structure'''
-The entry 7eg7 is ON HOLD
+==TFIID-based core PIC on SCP promoter==
+<StructureSection load='7eg7' size='340' side='right'caption='[[7eg7]], [[Resolution|resolution]] 6.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7eg7]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EG7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eg7 OCA], [https://pdbe.org/7eg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eg7 RCSB], [https://www.ebi.ac.uk/pdbsum/7eg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eg7 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.
-Authors: Chen, X., Qi, Y., Hou, H., Wang, X., Wu, Z., Li, J., Xu, Y.
+Structural insights into preinitiation complex assembly on core promoters.,Chen X, Qi Y, Wu Z, Wang X, Li J, Zhao D, Hou H, Li Y, Yu Z, Liu W, Wang M, Ren Y, Li Z, Yang H, Xu Y Science. 2021 Apr 30;372(6541):eaba8490. doi: 10.1126/science.aba8490. Epub 2021 , Apr 1. PMID:33795473<ref>PMID:33795473</ref>
-Description: TFIID-based core PIC on SCP promoter
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Chen, X]]
+<div class="pdbe-citations 7eg7" style="background-color:#fffaf0;"></div>
-[[Category: Hou, H]]
-[[Category: Li, J]]
+==See Also==
-[[Category: Wu, Z]]
+*[[Helicase 3D structures|Helicase 3D structures]]
-[[Category: Wang, X]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-[[Category: Qi, Y]]
+*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
-[[Category: Xu, Y]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Chen X]]
+[[Category: Hou H]]
+[[Category: Li J]]
+[[Category: Qi Y]]
+[[Category: Wang X]]
+[[Category: Wu Z]]
+[[Category: Xu Y]]