7ear: Difference between revisions

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-'''Unreleased structure'''
-The entry 7ear is ON HOLD
+==A positively charged mutant Cry3Aa endotoxin==
+<StructureSection load='7ear' size='340' side='right'caption='[[7ear]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7ear]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAR FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ear OCA], [https://pdbe.org/7ear PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ear RCSB], [https://www.ebi.ac.uk/pdbsum/7ear PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ear ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9S6N9_BACTU Q9S6N9_BACTU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Direct delivery of proteins into cells holds significant potential for basic research and drug development. However, the poor endosomal escape of conventional delivery strategies remains a challenge, thus limiting the clinical translation of many protein therapeutics. Herein, we report that engineered Cry3Aa protein (Pos3Aa) crystals formed naturally within Bacillus thuringiensis can serve as a vehicle for efficient cytosolic delivery of bioactive proteins. We showed that Pos3Aa-mediated delivery of tumor suppressor p53 protein, a promising therapeutic candidate found to be inactivated in nearly half of human cancers, resulted in the restoration of p53 function in p53-deficient cancer cells, and thereby sensitized them to 5-fluorouracil chemotherapy as demonstrated in in vitro and in vivo models. Our results validate that Pos3Aa crystals can be a robust and effective platform for the cytosolic delivery of effector proteins, and suggest that efficient uptake and endosomal escape could be critical for efficacious p53 protein-based cancer therapy.
-Authors:
+Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.,Yang Z, Lee MMM, Chan MK Biomaterials. 2021 Apr;271:120759. doi: 10.1016/j.biomaterials.2021.120759. Epub , 2021 Mar 16. PMID:33798968<ref>PMID:33798968</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7ear" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Pesticidal crystal protein|Pesticidal crystal protein]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bacillus thuringiensis]]
+[[Category: Large Structures]]
+[[Category: Chan MK]]
+[[Category: Lee MM]]
+[[Category: Yang Z]]