1ae9: Difference between revisions

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<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.  
[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input. DNA cleavage, strand exchange, and religation steps are linked by a covalent phosphotyrosine intermediate in which Tyr342 is attached to the 3'-phosphate of the DNA cut site. The 1.9 angstrom crystal structure of the integrase catalytic domain reveals a protein fold that is conserved in organisms ranging from archaebacteria to yeast and that suggests a model for interaction with target DNA. The attacking Tyr342 nucleophile is located on a flexible loop about 20 angstroms from a basic groove that contains all the other catalytically essential residues. This bipartite active site can account for several apparently paradoxical features of integrase family recombinases, including the capacity for both cis and trans cleavage of DNA.
Flexibility in DNA recombination: structure of the lambda integrase catalytic core.,Kwon HJ, Tirumalai R, Landy A, Ellenberger T Science. 1997 Apr 4;276(5309):126-31. PMID:9082984<ref>PMID:9082984</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ae9" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia virus Lambda]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ellenberger, T]]
[[Category: Ellenberger T]]
[[Category: Kwon, H J]]
[[Category: Kwon HJ]]
[[Category: Landy, A]]
[[Category: Landy A]]
[[Category: Tirumalai, R]]
[[Category: Tirumalai R]]
[[Category: Dna recombination]]
[[Category: Integrase]]
[[Category: Site-specific recombination]]

Latest revision as of 09:30, 7 February 2024

STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORESTRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE

Structural highlights

1ae9 is a 2 chain structure with sequence from Escherichia virus Lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VINT_LAMBD Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ae9, resolution 1.90Å

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