6zn4: Difference between revisions
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==MaeB malic enzyme domain apoprotein== | ==MaeB malic enzyme domain apoprotein== | ||
<StructureSection load='6zn4' size='340' side='right'caption='[[6zn4]]' scene=''> | <StructureSection load='6zn4' size='340' side='right'caption='[[6zn4]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6zn4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bdellovibrio_bacteriovorus_HD100 Bdellovibrio bacteriovorus HD100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZN4 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn4 OCA], [https://pdbe.org/6zn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn4 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn4 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.679Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zn4 OCA], [https://pdbe.org/6zn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zn4 RCSB], [https://www.ebi.ac.uk/pdbsum/6zn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zn4 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6MM15_BDEBA Q6MM15_BDEBA] [https://www.uniprot.org/uniprot/Q6MM14_BDEBA Q6MM14_BDEBA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial hybrid malic enzymes (MaeB grouping, multidomain) catalyse the transformation of malate to pyruvate, and are a major contributor to cellular reducing power and carbon flux. Distinct from other malic enzyme subtypes, the hybrid enzymes are regulated by acetyl-CoA, a molecular indicator of the metabolic state of the cell. Here we solve the structure of a MaeB protein, which reveals hybrid enzymes use the appended phosphotransacetylase (PTA) domain to form a hexameric sensor that communicates acetyl-CoA occupancy to the malic enzyme active site, 60 A away. We demonstrate that allostery is governed by a large-scale rearrangement that rotates the catalytic subunits 70 degrees between the two states, identifying MaeB as a new model enzyme for the study of ligand-induced conformational change. Our work provides the mechanistic basis for metabolic control of hybrid malic enzymes, and identifies inhibition-insensitive variants that may find utility in synthetic biology. | |||
A rotary mechanism for allostery in bacterial hybrid malic enzymes.,Harding CJ, Cadby IT, Moynihan PJ, Lovering AL Nat Commun. 2021 Feb 23;12(1):1228. doi: 10.1038/s41467-021-21528-2. PMID:33623032<ref>PMID:33623032</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zn4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bdellovibrio bacteriovorus HD100]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Harding CJ]] | [[Category: Harding CJ]] | ||
[[Category: Lovering AL]] | [[Category: Lovering AL]] | ||