1z93: Difference between revisions
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<StructureSection load='1z93' size='340' side='right'caption='[[1z93]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='1z93' size='340' side='right'caption='[[1z93]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1z93]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1z93]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z93 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z93 OCA], [https://pdbe.org/1z93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z93 RCSB], [https://www.ebi.ac.uk/pdbsum/1z93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z93 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CAH3_HUMAN CAH3_HUMAN] Reversible hydration of carbon dioxide. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 38: | Line 36: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Agbandje-McKenna | [[Category: Agbandje-McKenna M]] | ||
[[Category: An | [[Category: An H]] | ||
[[Category: Duda | [[Category: Duda DM]] | ||
[[Category: Fisher | [[Category: Fisher SZ]] | ||
[[Category: Govindasamy | [[Category: Govindasamy L]] | ||
[[Category: Laipis | [[Category: Laipis PJ]] | ||
[[Category: McKenna | [[Category: McKenna R]] | ||
[[Category: Silverman | [[Category: Silverman DN]] | ||
[[Category: Tu | [[Category: Tu C]] | ||
[[Category: Yoshioka | [[Category: Yoshioka C]] | ||
Latest revision as of 10:06, 23 August 2023
Human Carbonic Anhydrase III:Structural and Kinetic study of Catalysis and Proton Transfer.Human Carbonic Anhydrase III:Structural and Kinetic study of Catalysis and Proton Transfer.
Structural highlights
FunctionCAH3_HUMAN Reversible hydration of carbon dioxide. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe residue phenylalanine 198 (Phe 198) is a prominent cause of the lower activity of human carbonic anhydrase III (HCA III) compared with HCA II and other isozymes which have leucine at this site. We report the crystal structures of HCA III and the site-directed mutant F198L HCA III, both at 2.1 A resolution, and the enhancement of catalytic activity by exogenous proton donors containing imidazole rings. Both enzymes had a hexahistidine extension at the carboxy-terminal end, used to aid in purification, that was ordered in the crystal structures bound in the active site cavity of an adjacent symmetry-related enzyme. This observation allowed us to comment on a number of possible binding sites for imidazole and derivatives as exogenous proton donors/acceptors in catalysis by HCA III. Kinetic and structural evidence indicates that the phenyl side chain of Phe 198 in HCA III, about 5 A from the zinc, is a steric constriction in the active site, may cause altered interactions at the zinc-bound solvent, and is a binding site for the activation of catalysis by histidylhistidine. This suggests that sites of activation of the proton-transfer pathway in carbonic anhydrase are closer to the zinc than considered in previous studies. Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer.,Duda DM, Tu C, Fisher SZ, An H, Yoshioka C, Govindasamy L, Laipis PJ, Agbandje-McKenna M, Silverman DN, McKenna R Biochemistry. 2005 Aug 2;44(30):10046-53. PMID:16042381[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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