7b7u: Difference between revisions
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New page: '''Unreleased structure''' The entry 7b7u is ON HOLD Authors: Finau, I., Dienemann, C., Aibara, S., Schilbach, S., Cramer, P. Description: Structure of RNA polymerase II-RPAP2 complex ... |
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The | ==Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2== | ||
<StructureSection load='7b7u' size='340' side='right'caption='[[7b7u]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7b7u]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Sus_scrofa_domesticus Sus scrofa domesticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B7U FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b7u OCA], [https://pdbe.org/7b7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b7u RCSB], [https://www.ebi.ac.uk/pdbsum/7b7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b7u ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/I3LJR4_PIG I3LJR4_PIG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human RPAP2 at 2.8 A resolution. The structure shows that RPAP2 binds between the jaw domains of the polymerase subunits RPB1 and RPB5. RPAP2 is incompatible with binding of downstream DNA during transcription and is displaced upon formation of a transcription pre-initiation complex. | |||
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2.,Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P Commun Biol. 2021 May 21;4(1):606. doi: 10.1038/s42003-021-02088-z. PMID:34021257<ref>PMID:34021257</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7b7u" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Sus scrofa domesticus]] | |||
[[Category: Aibara S]] | |||
[[Category: Cramer P]] | |||
[[Category: Dienemann C]] | |||
[[Category: Fianu I]] | |||
[[Category: Schilbach S]] | |||
Latest revision as of 09:06, 21 November 2024
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2
Structural highlights
FunctionI3LJR4_PIG DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] Publication Abstract from PubMedNuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human RPAP2 at 2.8 A resolution. The structure shows that RPAP2 binds between the jaw domains of the polymerase subunits RPB1 and RPB5. RPAP2 is incompatible with binding of downstream DNA during transcription and is displaced upon formation of a transcription pre-initiation complex. Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2.,Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P Commun Biol. 2021 May 21;4(1):606. doi: 10.1038/s42003-021-02088-z. PMID:34021257[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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