1d54: Difference between revisions

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[[Image:1d54.jpg|left|200px]]


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==ANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCIN==
The line below this paragraph, containing "STRUCTURE_1d54", creates the "Structure Box" on the page.
<StructureSection load='1d54' size='340' side='right'caption='[[1d54]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1d54]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D54 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DM6:4-EPIDOXORUBICIN'>DM6</scene></td></tr>
{{STRUCTURE_1d54|  PDB=1d54  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d54 OCA], [https://pdbe.org/1d54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d54 RCSB], [https://www.ebi.ac.uk/pdbsum/1d54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d54 ProSAT]</span></td></tr>
 
</table>
'''ANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCIN'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Brown T]]
Crystallographic methods have been applied to determine the high-resolution structure of the complex formed between the self-complementary oligonucleotide d(TGTACA) and the anthracycline antibiotic 4'-epiadriamycin. The complex crystallises in the tetragonal system, space group P4(1)2(1)2 with a = 2.802 nm and c = 5.293 nm, and an asymmetric unit consisting of a single DNA strand, one drug molecule and 34 solvent molecules. The refinement converged with an R factor of 0.17 for the 2381 reflections with F greater than or equal to 3 sigma F in the resolution range 0.70-0.14 nm. Two asymmetric units associate such that a distorted B-DNA-type hexanucleotide duplex is formed incorporating two drug molecules that are intercalated at the TpG steps. The amino sugar of 4'-epiadriamycin binds in the minor groove of the duplex and displays different interactions from those observed in previously determined structures. Interactions between the hydrophilic groups of the amino sugar and the oligonucleotide are all mediated by solvent molecules. Ultraviolet melting measurements and comparison with other anthracycline-DNA complexes suggest that these indirect interactions have a powerful stabilising effect on the complex.
[[Category: Hunter WN]]
 
[[Category: Leonard GA]]
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D54 OCA].  
 
==Reference==
Anthracycline binding to DNA. High-resolution structure of d(TGTACA) complexed with 4'-epiadriamycin., Leonard GA, Brown T, Hunter WN, Eur J Biochem. 1992 Feb 15;204(1):69-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1740157 1740157]
[[Category: Brown, T.]]
[[Category: Hunter, W N.]]
[[Category: Leonard, G A.]]
[[Category: Complexed with drug]]
[[Category: Double helix]]
[[Category: Right handed dna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:27:51 2008''

Latest revision as of 09:49, 7 February 2024

ANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCINANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCIN

Structural highlights

1d54 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1d54, resolution 1.40Å

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