7dic: Difference between revisions
New page: '''Unreleased structure''' The entry 7dic is ON HOLD Authors: Abula, A., Quan, X., Li, X., Yang, T., Li, T., Chen, Q., Ji, X. Description: Mycoplasma genitalium RNase R in complex with... |
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==Mycoplasma genitalium RNase R in complex with single-stranded RNA== | |||
<StructureSection load='7dic' size='340' side='right'caption='[[7dic]], [[Resolution|resolution]] 2.24Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7dic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_genitalium_G37 Mycoplasma genitalium G37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DIC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.242Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dic OCA], [https://pdbe.org/7dic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dic RCSB], [https://www.ebi.ac.uk/pdbsum/7dic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dic ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RNR_MYCGE RNR_MYCGE] 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA 2'-O-methylation is widely distributed and plays important roles in various cellular processes. Mycoplasma genitalium RNase R (MgR), a prokaryotic member of the RNase II/RNB family, is a 3'-5' exoribonuclease and is particularly sensitive to RNA 2'-O-methylation. However, how RNase R interacts with various RNA species and exhibits remarkable sensitivity to substrate 2'-O-methyl modifications remains elusive. Here we report high-resolution crystal structures of MgR in apo form and in complex with various RNA substrates. The structural data together with extensive biochemical analysis quantitively illustrate MgR's ribonuclease activity and significant sensitivity to RNA 2'-O-methylation. Comparison to its related homologs reveals an exquisite mechanism for the recognition and degradation of RNA substrates. Through structural and mutagenesis studies, we identified proline 277 to be responsible for the significant sensitivity of MgR to RNA 2'-O-methylation within the RNase II/RNB family. We also generated several MgR variants with modulated activities. Our work provides a mechanistic understanding of MgR activity that can be harnessed as a powerful RNA analytical tool that will open up a new venue for RNA 2'-O-methylations research in biological and clinical samples. | |||
Molecular mechanism of RNase R substrate sensitivity for RNA ribose methylation.,Abula A, Li X, Quan X, Yang T, Liu Y, Guo H, Li T, Ji X Nucleic Acids Res. 2021 Mar 31. pii: 6206732. doi: 10.1093/nar/gkab202. PMID:33788943<ref>PMID:33788943</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7dic" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Ji | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Quan | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycoplasma genitalium G37]] | |||
[[Category: Abula A]] | |||
[[Category: Chen Q]] | |||
[[Category: Ji X]] | |||
[[Category: Li T]] | |||
[[Category: Li X]] | |||
[[Category: Quan X]] | |||
[[Category: Yang T]] | |||