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==NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli==
==NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli==
<StructureSection load='1e5u' size='340' side='right'caption='[[1e5u]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='1e5u' size='340' side='right'caption='[[1e5u]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1e5u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1e1b 1e1b] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1inm 1inm]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1E5U FirstGlance]. <br>
<table><tr><td colspan='2'>[[1e5u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1e1b 1e1b] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1inm 1inm]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E5U FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EAE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1e5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e5u OCA], [http://pdbe.org/1e5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e5u RCSB], [http://www.ebi.ac.uk/pdbsum/1e5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e5u ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e5u OCA], [https://pdbe.org/1e5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e5u RCSB], [https://www.ebi.ac.uk/pdbsum/1e5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e5u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/EAE_ECO27 EAE_ECO27]] Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.  
[https://www.uniprot.org/uniprot/EAE_ECO27 EAE_ECO27] Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e5u ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e5u ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Intimin is a bacterial adhesion molecule involved in intimate attachment of enteropathogenic and enterohaemorrhagic Escherichia coli to mammalian host cells. Intimin targets the translocated intimin receptor (Tir), which is exported by the bacteria and integrated into the host cell plasma membrane. In this study we localized the Tir-binding region of intimin to the C-terminal 190 amino acids (Int190). We have also determined the region's high-resolution solution structure, which comprises an immunoglobulin domain that is intimately coupled to a novel C-type lectin domain. This fragment, which is necessary and sufficient for Tir interaction, defines a new super domain in intimin that exhibits striking structural similarity to the integrin-binding domain of the Yersinia invasin and C-type lectin families. The extracellular portion of intimin comprises an articulated rod of immunoglobulin domains extending from the bacterium surface, conveying a highly accessible 'adhesive tip' to the target cell. The interpretation of NMR-titration and mutagenesis data has enabled us to identify, for the first time, the binding site for Tir, which is located at the extremity of the Int190 moiety.
Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic Escherichia coli.,Batchelor M, Prasannan S, Daniell S, Reece S, Connerton I, Bloomberg G, Dougan G, Frankel G, Matthews S EMBO J. 2000 Jun 1;19(11):2452-64. PMID:10835344<ref>PMID:10835344</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1e5u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Batchelor, M]]
[[Category: Batchelor M]]
[[Category: Bloomberg, G]]
[[Category: Bloomberg G]]
[[Category: Connerton, I]]
[[Category: Connerton I]]
[[Category: Daniell, S]]
[[Category: Daniell S]]
[[Category: Dougan, G]]
[[Category: Dougan G]]
[[Category: Frankel, G]]
[[Category: Frankel G]]
[[Category: Matthews, S J]]
[[Category: Matthews SJ]]
[[Category: Prasannan, S]]
[[Category: Prasannan S]]
[[Category: Reece, S]]
[[Category: Reece S]]
[[Category: Cell adhesion]]
[[Category: Escherichia coli]]
[[Category: Intimin]]
[[Category: Nmr spectroscopy]]
[[Category: Outer membrane protein]]

Latest revision as of 12:58, 20 March 2024

NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coliNMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli

Structural highlights

1e5u is a 1 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 1e1b and 1inm. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EAE_ECO27 Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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