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| <!--
| | ==CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG== |
| The line below this paragraph, containing "STRUCTURE_1d29", creates the "Structure Box" on the page.
| | <StructureSection load='1d29' size='340' side='right'caption='[[1d29]], [[Resolution|resolution]] 2.50Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1d29]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D29 FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| -->
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d29 OCA], [https://pdbe.org/1d29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d29 RCSB], [https://www.ebi.ac.uk/pdbsum/1d29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d29 ProSAT]</span></td></tr> |
| {{STRUCTURE_1d29| PDB=1d29 | SCENE= }}
| | </table> |
| | | __TOC__ |
| '''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| | | [[Category: Dickerson RE]] |
| ==Overview== | | [[Category: Kopka ML]] |
| The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.
| | [[Category: Larsen TA]] |
| | |
| ==About this Structure==
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| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. | |
| | |
| ==Reference== | |
| Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1850624 1850624]
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| [[Category: Dickerson, R E.]] | |
| [[Category: Kopka, M L.]] | |
| [[Category: Larsen, T A.]] | |
| [[Category: B-dna]] | |
| [[Category: Double helix]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:22:02 2008''
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