Shiga toxin: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Max Evoy-Mount, Michal Harel, Alexander Berchansky

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 Shiga Toxin acts as an N-glycosidase, removing an adenine from the 28S ribosomal rRNA of a target cell which leads to inhibition of protein elongation and ultimately cellular apoptosis.<ref name=Di>PMID: 21184769</ref>  The B subunit is necessary for binding to globo series glycolipid globotriaosylceramide (Gb<sub>3</sub>), a eukaryotic membrane receptor, where it is then endocytosed and proteolytically cleaved into an active A subunit and a B subunit.<ref name=Lenz>PMID: 2170899</ref>  The B subunit is not active in the depurination of of 28S rRNA, but is essential for GB<sub>3</sub> binding and therefore essential for toxicity.  Once in the cytosol the A subunit is free to interact with and inactivate 28S rRNA.  On the A subunit <scene name='Shiga_toxin_1/Active_site_zoomed_in/1'>Tyr77, Tyr114, Glu167, Arg170, and Trp203</scene> are all essential in glycosidic activity.<ref name=Di>PMID: 21184769</ref>  This mechanism (B subunit binding to globotriaosylceramide and A subunit depurinating 28S rRNA) is conserved amongst the Stx family as well as the ricin toxin.
-</StructureSection>
-__NOTOC__
 ==3D structures of shiga toxin==
+[[Shiga toxin 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*Shiga toxin
-**[[1dm0]], [[1r4q]] – SdStx α+β chains – ''Shigella dysenteriae''<br />
-**[[4m1u]] – EcStx2 α+β chains + disaccharide – ''Escherichia coli''<br />
-**[[6u3u]] – EcStx 2K α (mutant) +β chains <br />
-*Shiga-like toxin 1
-**[[1czw]], [[1czg]] – pHStx1 β chain (mutant) – phage H30<br />
-**[[4ull]] – pHStx1 β chain – NMR<br />
-**[[1c48]] - pHStx1 β chain receptor-binding domain (mutant)<br />
-**[[1bos]], [[1qnu]] – pHStx1 β chain receptor-binding domain + GB3 analog<br />
-**[[2xsc]] – EcStx1 β chain <br />
-**[[1d1i]], [[1cqf]] – EcStx1 β chain (mutant) + GB3 analog <br />
-**[[1c4q]], [[1d1k]] – EcStx1 β chain receptor-binding domain (mutant) + GB3 analog
-*Shiga-like toxin 2
-**[[2c5c]] - pHStx2 β chain + inhibitor<br />
-**[[1r4p]] – EcStx2 α+β chains<br />
-**[[3mxg]] – EcStx2 β chain (mutant)<br />
-**[[4p2c]] - EcStx2 α+β chains (mutant) + nanobody<br />
-**[[2bos]], [[1qoh]] – EcStx2 β chain receptor-binding domain (mutant) + GB3 analog<br />
-**[[2ga4]] - epStx2 α+β chains – enterobacteria phage 933W<br />
-**[[6fe4]] - epStx2 β chain +NB113<br />
-}}
 ==References==
 {{Reflist}}
 [[Category:Topic Page]]