7k5x: Difference between revisions

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-'''Unreleased structure'''
-The entry 7k5x is ON HOLD  until Paper Publication
+==Cryo-EM structure of a chromatosome containing human linker histone H1.0==
+<StructureSection load='7k5x' size='340' side='right'caption='[[7k5x]], [[Resolution|resolution]] 2.93&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7k5x]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K5X FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.93&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k5x OCA], [https://pdbe.org/7k5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k5x RCSB], [https://www.ebi.ac.uk/pdbsum/7k5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k5x ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1. There are 11 human H1 isoforms with diverse cellular functions, but how they interact with the nucleosome remains elusive. Here, we determined the cryoelectron microscopy (cryo-EM) structures of chromatosomes containing 197 bp DNA and three different human H1 isoforms, respectively. The globular domains of all three H1 isoforms bound to the nucleosome dyad. However, the flanking/linker DNAs displayed substantial distinct dynamic conformations. Nuclear magnetic resonance (NMR) and H1 tail-swapping cryo-EM experiments revealed that the C-terminal tails of the H1 isoforms mainly controlled the flanking DNA orientations. We also observed partial ordering of the core histone H2A C-terminal and H3 N-terminal tails in the chromatosomes. Our results provide insights into the structures and dynamics of the chromatosomes and have implications for the structure and function of chromatin.
-Authors:
+Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.,Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y Mol Cell. 2021 Jan 7;81(1):166-182.e6. doi: 10.1016/j.molcel.2020.10.038. Epub , 2020 Nov 24. PMID:33238161<ref>PMID:33238161</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7k5x" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[Histone 3D structures|Histone 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Bai Y]]
+[[Category: Zhou B-R]]