5xt2: Difference between revisions

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<StructureSection load='5xt2' size='340' side='right'caption='[[5xt2]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
<StructureSection load='5xt2' size='340' side='right'caption='[[5xt2]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5xt2]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XT2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XT2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5xt2]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XT2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.652&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xso|5xso]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xt2 OCA], [http://pdbe.org/5xt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xt2 RCSB], [http://www.ebi.ac.uk/pdbsum/5xt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xt2 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xt2 OCA], [https://pdbe.org/5xt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xt2 RCSB], [https://www.ebi.ac.uk/pdbsum/5xt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xt2 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The symbiotic nitrogen-fixing bacterium Bradyrhizobium japonicum is critical to the agro-industrial production of soybean because it enables the production of high yields of soybeans with little use of nitrogenous fertilizers. The FixL and FixJ two-component system (TCS) of this bacterium ensures that nitrogen fixation is only stimulated under conditions of low oxygen. When it is not bound to oxygen, the histidine kinase FixL undergoes autophosphorylation and transfers phosphate from adenosine triphosphate (ATP) to the response regulator FixJ, which, in turn, stimulates the expression of genes required for nitrogen fixation. We purified full-length B. japonicum FixL and FixJ proteins and defined their structures individually and in complex using small-angle x-ray scattering, crystallographic, and in silico modeling techniques. Comparison of active and inactive forms of FixL suggests that intramolecular signal transduction is driven by local changes in the sensor domain and in the coiled-coil region connecting the sensor and histidine kinase domains. We also found that FixJ exhibits conformational plasticity not only in the monomeric state but also in tetrameric complexes with FixL during phosphotransfer. This structural characterization of a complete TCS contributes both a mechanistic and evolutionary understanding to TCS signal relay, specifically in the context of the control of nitrogen fixation in root nodules.
Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system.,Wright GSA, Saeki A, Hikima T, Nishizono Y, Hisano T, Kamaya M, Nukina K, Nishitani H, Nakamura H, Yamamoto M, Antonyuk SV, Hasnain SS, Shiro Y, Sawai H Sci Signal. 2018 Apr 10;11(525). pii: 11/525/eaaq0825. doi:, 10.1126/scisignal.aaq0825. PMID:29636388<ref>PMID:29636388</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xt2" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Response regulator 3D structure|Response regulator 3D structure]]
*[[Response regulator 3D structure|Response regulator 3D structure]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bradyrhizobium japonicum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Antonyuk, S V]]
[[Category: Antonyuk SV]]
[[Category: Hasnain, S S]]
[[Category: Hasnain SS]]
[[Category: Hikima, T]]
[[Category: Hikima T]]
[[Category: Hisano, T]]
[[Category: Hisano T]]
[[Category: Nakamura, H]]
[[Category: Nakamura H]]
[[Category: Nishizono, Y]]
[[Category: Nishizono Y]]
[[Category: Saeki, A]]
[[Category: Saeki A]]
[[Category: Sawai, H]]
[[Category: Sawai H]]
[[Category: Shiro, Y]]
[[Category: Shiro Y]]
[[Category: Wright, G S.A]]
[[Category: Wright GSA]]
[[Category: Yamamoto, M]]
[[Category: Yamamoto M]]
[[Category: Dna binding protein]]
[[Category: Fixj/narl family]]
[[Category: Full-length]]
[[Category: Response regulater]]
[[Category: Unphosphorylated monomeric state]]

Latest revision as of 13:20, 27 March 2024

Crystal structures of full-length FixJ from B. japonicum crystallized in space group P212121Crystal structures of full-length FixJ from B. japonicum crystallized in space group P212121

Structural highlights

5xt2 is a 5 chain structure with sequence from Bradyrhizobium japonicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.652Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

5xt2, resolution 2.65Å

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