6vqj: Difference between revisions

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<StructureSection load='6vqj' size='340' side='right'caption='[[6vqj]], [[Resolution|resolution]] 5.70&Aring;' scene=''>
<StructureSection load='6vqj' size='340' side='right'caption='[[6vqj]], [[Resolution|resolution]] 5.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6vqj]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VQJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[6vqj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VQJ FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqj OCA], [http://pdbe.org/6vqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vqj RCSB], [http://www.ebi.ac.uk/pdbsum/6vqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqj ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqj OCA], [https://pdbe.org/6vqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vqj RCSB], [https://www.ebi.ac.uk/pdbsum/6vqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/VPP1_RAT VPP1_RAT]] Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity). [[http://www.uniprot.org/uniprot/VATC1_RAT VATC1_RAT]] Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (By similarity). [[http://www.uniprot.org/uniprot/Q8R2H0_RAT Q8R2H0_RAT]] Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.[RuleBase:RU364019] [[http://www.uniprot.org/uniprot/VATE1_RAT VATE1_RAT]] Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
[https://www.uniprot.org/uniprot/VATC1_RAT VATC1_RAT] Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes possess numerous subunit isoforms, which complicates their analysis. We isolated homogeneous rat brain V-ATPase through its interaction with SidK, a Legionella pneumophila effector protein. Cryo-electron microscopy allowed the construction of an atomic model, defining the enzyme's ATP:proton ratio as 3:10 and revealing a homolog of yeast subunit f in the membrane region, which we tentatively identify as RNAseK. The c ring encloses the transmembrane anchors for cleaved ATP6AP1/Ac45 and ATP6AP2/PRR, the latter of which is the (pro)renin receptor that, in other contexts, is involved in both Wnt signaling and the renin-angiotensin system that regulates blood pressure. This structure shows how ATP6AP1/Ac45 and ATP6AP2/PRR enable assembly of the enzyme's catalytic and membrane regions.
 
Structure of V-ATPase from the mammalian brain.,Abbas YM, Wu D, Bueler SA, Robinson CV, Rubinstein JL Science. 2020 Mar 13;367(6483):1240-1246. doi: 10.1126/science.aaz2924. PMID:32165585<ref>PMID:32165585</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vqj" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Abbas, Y M]]
[[Category: Abbas YM]]
[[Category: Rubinstein, J L]]
[[Category: Rubinstein JL]]
[[Category: Membrane protein complex]]
[[Category: Proton transport]]
[[Category: Rotary atpase]]

Latest revision as of 17:39, 6 March 2024

Mammalian V-ATPase from rat brain collar and peripheral stalks rotational state 2 (from focused refinement)Mammalian V-ATPase from rat brain collar and peripheral stalks rotational state 2 (from focused refinement)

Structural highlights

6vqj is a 8 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 5.7Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VATC1_RAT Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (By similarity).

See Also

6vqj, resolution 5.70Å

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