7d1a: Difference between revisions
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==cryo-EM structure of a group II intron RNP complexed with its reverse transcriptase== | |||
<StructureSection load='7d1a' size='340' side='right'caption='[[7d1a]], [[Resolution|resolution]] 3.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7d1a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_cremoris Lactococcus cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D1A FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | |||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d1a OCA], [https://pdbe.org/7d1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d1a RCSB], [https://www.ebi.ac.uk/pdbsum/7d1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d1a ProSAT]</span></td></tr> | ||
[[Category: | </table> | ||
[[Category: | == Function == | ||
[[Category: | [https://www.uniprot.org/uniprot/LTRA_LACLC LTRA_LACLC] Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by facilitating the formation of the catalytically active structure of the intron RNA. After splicing, the protein remains bound to the excised intron lariat RNA, forming ribonucleoprotein particles, and cleaving the antisense strand of the recipient DNA in the 3' exon. After DNA cleavage, retrohoming occurs by a target DNA-primed reverse transcription of the intron RNA that had reverse spliced into the sense strand of the recipient DNA. It also contributes to the recognition of the DNA target site and acts as a repressor of its own translation. | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Wang | [[Category: Lactococcus cremoris]] | ||
[[Category: Wang | [[Category: Large Structures]] | ||
[[Category: | [[Category: Belfort M]] | ||
[[Category: Dong XL]] | |||
[[Category: Hu CX]] | |||
[[Category: Liu N]] | |||
[[Category: Wang HW]] | |||
[[Category: Wang J]] | |||
[[Category: Wang JW]] | |||
[[Category: Zeng JW]] | |||
Latest revision as of 13:52, 27 March 2024
cryo-EM structure of a group II intron RNP complexed with its reverse transcriptasecryo-EM structure of a group II intron RNP complexed with its reverse transcriptase
Structural highlights
FunctionLTRA_LACLC Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by facilitating the formation of the catalytically active structure of the intron RNA. After splicing, the protein remains bound to the excised intron lariat RNA, forming ribonucleoprotein particles, and cleaving the antisense strand of the recipient DNA in the 3' exon. After DNA cleavage, retrohoming occurs by a target DNA-primed reverse transcription of the intron RNA that had reverse spliced into the sense strand of the recipient DNA. It also contributes to the recognition of the DNA target site and acts as a repressor of its own translation. |
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