7crt: Difference between revisions

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-'''Unreleased structure'''
-The entry 7crt is ON HOLD
+==Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif 100 ps after light activation (0.17mJ/mm2)==
+<StructureSection load='7crt' size='340' side='right'caption='[[7crt]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crt OCA], [https://pdbe.org/7crt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crt RCSB], [https://www.ebi.ac.uk/pdbsum/7crt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crt ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chloride ion-pumping rhodopsin (ClR) in some marine bacteria utilizes light energy to actively transport Cl(-) into cells. How the ClR initiates the transport is elusive. Here, we show the dynamics of ion transport observed with time-resolved serial femtosecond (fs) crystallography using the Linac Coherent Light Source. X-ray pulses captured structural changes in ClR upon flash illumination with a 550 nm fs-pumping laser. High-resolution structures for five time points (dark to 100 ps after flashing) reveal complex and coordinated dynamics comprising retinal isomerization, water molecule rearrangement, and conformational changes of various residues. Combining data from time-resolved spectroscopy experiments and molecular dynamics simulations, this study reveals that the chloride ion close to the Schiff base undergoes a dissociation-diffusion process upon light-triggered retinal isomerization.
-Authors: Yun, J.H., Liu, H., Lee, W.T., Schmidt, M.
+Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.,Yun JH, Li X, Yue J, Park JH, Jin Z, Li C, Hu H, Shi Y, Pandey S, Carbajo S, Boutet S, Hunter MS, Liang M, Sierra RG, Lane TJ, Zhou L, Weierstall U, Zatsepin NA, Ohki M, Tame JRH, Park SY, Spence JCH, Zhang W, Schmidt M, Lee W, Liu H Proc Natl Acad Sci U S A. 2021 Mar 30;118(13). pii: 2020486118. doi:, 10.1073/pnas.2020486118. PMID:33753488<ref>PMID:33753488</ref>
-Description: Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif 100 ps after light activation (0.17mJ/mm2)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Schmidt, M]]
+<div class="pdbe-citations 7crt" style="background-color:#fffaf0;"></div>
-[[Category: Lee, W.T]]
-[[Category: Yun, J.H]]
+==See Also==
-[[Category: Liu, H]]
+*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Lee WT]]
+[[Category: Liu H]]
+[[Category: Schmidt M]]
+[[Category: Yun JH]]