5kvf: Difference between revisions
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<StructureSection load='5kvf' size='340' side='right'caption='[[5kvf]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='5kvf' size='340' side='right'caption='[[5kvf]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5kvf]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5kvf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KVF FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvf OCA], [https://pdbe.org/5kvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kvf RCSB], [https://www.ebi.ac.uk/pdbsum/5kvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvf ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0X8GJ44_ZIKV A0A0X8GJ44_ZIKV] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Zika virus]] | [[Category: Zika virus]] | ||
[[Category: Fremont DH]] | |||
[[Category: Nelson CA]] | |||
[[Category: Zhao H]] | |||
Latest revision as of 13:56, 27 September 2023
Zika specific antibody, ZV-64, bound to ZIKA envelope DIIIZika specific antibody, ZV-64, bound to ZIKA envelope DIII
Structural highlights
FunctionPublication Abstract from PubMedZika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. Here, we developed six mouse monoclonal antibodies (mAbs) against ZIKV including four (ZV-48, ZV-54, ZV-64, and ZV-67) that were ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. X-ray crystallographic and competition binding analyses of Fab fragments and scFvs defined three spatially distinct epitopes in DIII of the envelope protein corresponding to the lateral ridge (ZV-54 and ZV-67), C-C' loop (ZV-48 and ZV-64), and ABDE sheet (ZV-2) regions. In vivo passive transfer studies revealed protective activity of DIII-lateral ridge specific neutralizing mAbs in a mouse model of ZIKV infection. Our results suggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity, which provides a path for developing prophylactic antibodies for use in pregnancy or designing epitope-specific vaccines against ZIKV. Structural Basis of Zika Virus-Specific Antibody Protection.,Zhao H, Fernandez E, Dowd KA, Speer SD, Platt DJ, Gorman MJ, Govero J, Nelson CA, Pierson TC, Diamond MS, Fremont DH Cell. 2016 Jul 26. pii: S0092-8674(16)30927-8. doi: 10.1016/j.cell.2016.07.020. PMID:27475895[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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