2x25: Difference between revisions
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<StructureSection load='2x25' size='340' side='right'caption='[[2x25]], [[Resolution|resolution]] 1.20Å' scene=''> | <StructureSection load='2x25' size='340' side='right'caption='[[2x25]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2x25]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2x25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X25 FirstGlance]. <br> | ||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vbs|1vbs]], [[1oca|1oca]], [[1mf8|1mf8]], [[2cyh|2cyh]], [[1cwb|1cwb]], [[1vbt|1vbt]], [[1m9e|1m9e]], [[1cwl|1cwl]], [[1cwc|1cwc]], [[1cwi|1cwi]], [[1cwo|1cwo]], [[1rmh|1rmh]], [[1cwj|1cwj]], [[2rmb|2rmb]], [[1m9c|1m9c]], [[1cwa|1cwa]], [[1cwf|1cwf]], [[1m9y|1m9y]], [[3cyh|3cyh]], [[1m9f|1m9f]], [[4cyh|4cyh]], [[1cwh|1cwh]], [[1bck|1bck]], [[1w8v|1w8v]], [[1awr|1awr]], [[1nmk|1nmk]], [[1mik|1mik]], [[1awv|1awv]], [[1m9d|1m9d]], [[1awt|1awt]], [[2cpl|2cpl]], [[1fgl|1fgl]], [[1m9x|1m9x]], [[1m63|1m63]], [[1cwk|1cwk]], [[5cyh|5cyh]], [[1ak4|1ak4]], [[3cys|3cys]], [[2alf|2alf]], [[1aws|1aws]], [[1w8l|1w8l]], [[1awu|1awu]], [[1w8m|1w8m]], [[2rma|2rma]], [[1cwm|1cwm]], [[1awq|1awq]], [[2x2d|2x2d]], [[2x2a|2x2a]], [[2x2c|2x2c]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1vbs|1vbs]], [[1oca|1oca]], [[1mf8|1mf8]], [[2cyh|2cyh]], [[1cwb|1cwb]], [[1vbt|1vbt]], [[1m9e|1m9e]], [[1cwl|1cwl]], [[1cwc|1cwc]], [[1cwi|1cwi]], [[1cwo|1cwo]], [[1rmh|1rmh]], [[1cwj|1cwj]], [[2rmb|2rmb]], [[1m9c|1m9c]], [[1cwa|1cwa]], [[1cwf|1cwf]], [[1m9y|1m9y]], [[3cyh|3cyh]], [[1m9f|1m9f]], [[4cyh|4cyh]], [[1cwh|1cwh]], [[1bck|1bck]], [[1w8v|1w8v]], [[1awr|1awr]], [[1nmk|1nmk]], [[1mik|1mik]], [[1awv|1awv]], [[1m9d|1m9d]], [[1awt|1awt]], [[2cpl|2cpl]], [[1fgl|1fgl]], [[1m9x|1m9x]], [[1m63|1m63]], [[1cwk|1cwk]], [[5cyh|5cyh]], [[1ak4|1ak4]], [[3cys|3cys]], [[2alf|2alf]], [[1aws|1aws]], [[1w8l|1w8l]], [[1awu|1awu]], [[1w8m|1w8m]], [[2rma|2rma]], [[1cwm|1cwm]], [[1awq|1awq]], [[2x2d|2x2d]], [[2x2a|2x2a]], [[2x2c|2x2c]]</div></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x25 OCA], [https://pdbe.org/2x25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x25 RCSB], [https://www.ebi.ac.uk/pdbsum/2x25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x25 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/PPIA_HUMAN PPIA_HUMAN]] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Latest revision as of 13:44, 13 April 2022
Free acetyl-CypA orthorhombic formFree acetyl-CypA orthorhombic form
Structural highlights
Function[PPIA_HUMAN] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCyclophilin A (CypA) is a ubiquitous cis-trans prolyl isomerase with key roles in immunity and viral infection. CypA suppresses T-cell activation through cyclosporine complexation and is required for effective HIV-1 replication in host cells. We show that CypA is acetylated in diverse human cell lines and use a synthetically evolved acetyllysyl-tRNA synthetase/tRNA(CUA) pair to produce recombinant acetylated CypA in Escherichia coli. We determined atomic-resolution structures of acetylated CypA and its complexes with cyclosporine and HIV-1 capsid. Acetylation markedly inhibited CypA catalysis of cis to trans isomerization and stabilized cis rather than trans forms of the HIV-1 capsid. Furthermore, CypA acetylation antagonized the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition. Our results reveal that acetylation regulates key functions of CypA in immunity and viral infection and provide a general set of mechanisms by which acetylation modulates interactions to regulate cell function. Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization.,Lammers M, Neumann H, Chin JW, James LC Nat Chem Biol. 2010 May;6(5):331-7. Epub 2010 Apr 4. PMID:20364129[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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