6x8m: Difference between revisions

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==CryoEM structure of the holo-SrpI encapsulin complex from Synechococcus elongatus PCC 7942==
==CryoEM structure of the holo-SrpI encapsulin complex from Synechococcus elongatus PCC 7942==
<StructureSection load='6x8m' size='340' side='right'caption='[[6x8m]]' scene=''>
<StructureSection load='6x8m' size='340' side='right'caption='[[6x8m]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X8M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X8M FirstGlance]. <br>
<table><tr><td colspan='2'>[[6x8m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X8M FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8m OCA], [http://pdbe.org/6x8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x8m RCSB], [http://www.ebi.ac.uk/pdbsum/6x8m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8m ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8m OCA], [https://pdbe.org/6x8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x8m RCSB], [https://www.ebi.ac.uk/pdbsum/6x8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8m ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENCP2_SYNE7 ENCP2_SYNE7] Shell component of a type 2A encapsulin nanocompartment. Expression in E.coli generates nanocompartments with an average diameter of 25 nm. They can be disassembled by treatment with 6M guanidine hydrochloride and reassembled with cargo. The nanocompartment is probably involved in sulfur metabolism (PubMed:33821786). Probably allows passage of cysteine into its interior; during growth in light the physiological pH is 8-8.4, about 30-54% of free cysteine (charge -1) would be able to pass through the shell (Probable).<ref>PMID:33821786</ref> <ref>PMID:33821786</ref>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
[[Category: Bischoff AJ]]
[[Category: Bischoff AJ]]
[[Category: LaFrance BJ]]
[[Category: LaFrance BJ]]

Latest revision as of 17:48, 6 March 2024

CryoEM structure of the holo-SrpI encapsulin complex from Synechococcus elongatus PCC 7942CryoEM structure of the holo-SrpI encapsulin complex from Synechococcus elongatus PCC 7942

Structural highlights

6x8m is a 1 chain structure with sequence from Synechococcus elongatus PCC 7942 = FACHB-805. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENCP2_SYNE7 Shell component of a type 2A encapsulin nanocompartment. Expression in E.coli generates nanocompartments with an average diameter of 25 nm. They can be disassembled by treatment with 6M guanidine hydrochloride and reassembled with cargo. The nanocompartment is probably involved in sulfur metabolism (PubMed:33821786). Probably allows passage of cysteine into its interior; during growth in light the physiological pH is 8-8.4, about 30-54% of free cysteine (charge -1) would be able to pass through the shell (Probable).[1] [2]

References

  1. ↑ Nichols RJ, LaFrance B, Phillips NR, Radford DR, Oltrogge LM, Valentin-Alvarado LE, Bischoff AJ, Nogales E, Savage DF. Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism. Elife. 2021 Apr 6;10. pii: 59288. doi: 10.7554/eLife.59288. PMID:33821786 doi:http://dx.doi.org/10.7554/eLife.59288
  2. ↑ Nichols RJ, LaFrance B, Phillips NR, Radford DR, Oltrogge LM, Valentin-Alvarado LE, Bischoff AJ, Nogales E, Savage DF. Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism. Elife. 2021 Apr 6;10. pii: 59288. doi: 10.7554/eLife.59288. PMID:33821786 doi:http://dx.doi.org/10.7554/eLife.59288

6x8m, resolution 2.20Å

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