7c38: Difference between revisions
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==Crystal structure of AofleA from Arthrobotrys oligospora in complex with L-fucose== | |||
<StructureSection load='7c38' size='340' side='right'caption='[[7c38]], [[Resolution|resolution]] 1.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7c38]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Orbilia_oligospora_ATCC_24927 Orbilia oligospora ATCC 24927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C38 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c38 OCA], [https://pdbe.org/7c38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c38 RCSB], [https://www.ebi.ac.uk/pdbsum/7c38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c38 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G1XA82_ARTOA G1XA82_ARTOA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fungal lectin can bind specific carbohydrate structures of the host and work in recognition and adhesion or as a toxic factor. AofleA, as a fucose-specific lectin from widely studied nematode predatory fungus Arthrobotrys oligospora, possibly plays a key role in the event of capturing nematodes, but the mechanism remains unknown. Here we report the crystal structure of AofleA, which exists as a homodimer with each subunit folds as a six-bladed beta-propeller. Our structural and biological results revealed that three of the six putative binding sites of AofleA had fucose-binding abilities. In addition, we found that AofleA could bind to the pharynx and intestine of the nematode in a fucose-binding-dependent manner. Our results facilitate the understanding of the mechanism that fucose-specific lectin mediates fungi-nematodes interaction, and provide structural information for the development of potential applications of AofleA. | |||
Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.,Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064<ref>PMID:32698064</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7c38" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Orbilia oligospora ATCC 24927]] | |||
[[Category: Cheng X]] | |||
[[Category: Liu M]] | |||
[[Category: Wang J]] | |||
[[Category: Wang M]] | |||
[[Category: Zhang M]] | |||
Latest revision as of 18:53, 29 November 2023
Crystal structure of AofleA from Arthrobotrys oligospora in complex with L-fucoseCrystal structure of AofleA from Arthrobotrys oligospora in complex with L-fucose
Structural highlights
FunctionPublication Abstract from PubMedFungal lectin can bind specific carbohydrate structures of the host and work in recognition and adhesion or as a toxic factor. AofleA, as a fucose-specific lectin from widely studied nematode predatory fungus Arthrobotrys oligospora, possibly plays a key role in the event of capturing nematodes, but the mechanism remains unknown. Here we report the crystal structure of AofleA, which exists as a homodimer with each subunit folds as a six-bladed beta-propeller. Our structural and biological results revealed that three of the six putative binding sites of AofleA had fucose-binding abilities. In addition, we found that AofleA could bind to the pharynx and intestine of the nematode in a fucose-binding-dependent manner. Our results facilitate the understanding of the mechanism that fucose-specific lectin mediates fungi-nematodes interaction, and provide structural information for the development of potential applications of AofleA. Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.,Liu M, Cheng X, Wang J, Tian D, Tang K, Xu T, Zhang M, Wang Y, Wang M Int J Biol Macromol. 2020 Jul 19;164:783-793. doi:, 10.1016/j.ijbiomac.2020.07.173. PMID:32698064[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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