6pyt: Difference between revisions

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<StructureSection load='6pyt' size='340' side='right'caption='[[6pyt]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='6pyt' size='340' side='right'caption='[[6pyt]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6pyt]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PYT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PYT FirstGlance]. <br>
<table><tr><td colspan='2'>[[6pyt]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PYT FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pyt OCA], [http://pdbe.org/6pyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pyt RCSB], [http://www.ebi.ac.uk/pdbsum/6pyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pyt ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pyt OCA], [https://pdbe.org/6pyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pyt RCSB], [https://www.ebi.ac.uk/pdbsum/6pyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pyt ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9I5S9_PSEAE Q9I5S9_PSEAE]
R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1-4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage(5). Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
 
Action of a minimal contractile bactericidal nanomachine.,Ge P, Scholl D, Prokhorov NS, Avaylon J, Shneider MM, Browning C, Buth SA, Plattner M, Chakraborty U, Ding K, Leiman PG, Miller JF, Zhou ZH Nature. 2020 Apr;580(7805):658-662. doi: 10.1038/s41586-020-2186-z. Epub 2020 Apr, 15. PMID:32350467<ref>PMID:32350467</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6pyt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pseae]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Avaylon, J]]
[[Category: Avaylon J]]
[[Category: Browning, C]]
[[Category: Browning C]]
[[Category: Buth, S A]]
[[Category: Buth SA]]
[[Category: Ding, K]]
[[Category: Ding K]]
[[Category: Ge, P]]
[[Category: Ge P]]
[[Category: Leiman, P G]]
[[Category: Leiman PG]]
[[Category: Miller, J F]]
[[Category: Miller JF]]
[[Category: Plattner, M]]
[[Category: Plattner M]]
[[Category: Scholl, D]]
[[Category: Scholl D]]
[[Category: Shneider, M M]]
[[Category: Shneider MM]]
[[Category: Zhou, Z H]]
[[Category: Zhou ZH]]
[[Category: Antimicrobial protein]]
[[Category: Bacteriocin]]
[[Category: Helix]]
[[Category: Pyocin]]

Latest revision as of 12:27, 20 March 2024

CryoEM Structure of Pyocin R2 - precontracted - trunkCryoEM Structure of Pyocin R2 - precontracted - trunk

Structural highlights

6pyt is a 24 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9I5S9_PSEAE

6pyt, resolution 2.90Å

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OCA
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