5fb6: Difference between revisions
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<StructureSection load='5fb6' size='340' side='right'caption='[[5fb6]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='5fb6' size='340' side='right'caption='[[5fb6]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5fb6]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5fb6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FB6 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.901Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fb6 OCA], [https://pdbe.org/5fb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fb6 RCSB], [https://www.ebi.ac.uk/pdbsum/5fb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fb6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
[[Category: Burkhardt | [[Category: Burkhardt A]] | ||
[[Category: David | [[Category: David C]] | ||
[[Category: Duman | [[Category: Duman R]] | ||
[[Category: Meents | [[Category: Meents A]] | ||
[[Category: Roedig | [[Category: Roedig P]] | ||
[[Category: Sanchez-Weatherby | [[Category: Sanchez-Weatherby J]] | ||
[[Category: Vartiainen | [[Category: Vartiainen I]] | ||
[[Category: Wagner | [[Category: Wagner A]] | ||
[[Category: Warmer | [[Category: Warmer M]] | ||
Latest revision as of 09:44, 19 July 2023
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scatteringRoom-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
Structural highlights
FunctionINS_PIG Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Publication Abstract from PubMedRecent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector technologies with high framing rates up to 1 kHz, fast sample exchange has become the bottleneck for such experiments. A micro-patterned chip has been developed from single-crystalline silicon, which acts as a sample holder for up to several thousand microcrystals at a very low background level. The crystals can be easily loaded onto the chip and excess mother liquor can be efficiently removed. Dehydration of the crystals is prevented by keeping them in a stream of humidified air during data collection. Further sealing of the sample holder, for example with Kapton, is not required. Room-temperature data collection from insulin crystals loaded onto the chip proves the applicability of the chip for macromolecular crystallography. Subsequent structure refinements reveal no radiation-damage-induced structural changes for insulin crystals up to a dose of 565.6 kGy, even though the total diffraction power of the crystals has on average decreased to 19.1% of its initial value for the same dose. A decay of the diffracting power by half is observed for a dose of D 1/2 = 147.5 +/- 19.1 kGy, which is about 1/300 of the dose before crystals show a similar decay at cryogenic temperatures. Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering.,Roedig P, Duman R, Sanchez-Weatherby J, Vartiainen I, Burkhardt A, Warmer M, David C, Wagner A, Meents A J Appl Crystallogr. 2016 May 23;49(Pt 3):968-975. eCollection 2016 Jun 1. PMID:27275143[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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