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| <SX load='6v6b' size='340' side='right' viewer='molstar' caption='[[6v6b]], [[Resolution|resolution]] 3.80Å' scene=''> | | <SX load='6v6b' size='340' side='right' viewer='molstar' caption='[[6v6b]], [[Resolution|resolution]] 3.80Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6v6b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V6B FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6v6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V6B FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6b OCA], [http://pdbe.org/6v6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6b RCSB], [http://www.ebi.ac.uk/pdbsum/6v6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6b ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6b OCA], [https://pdbe.org/6v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v6b RCSB], [https://www.ebi.ac.uk/pdbsum/6v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6b ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/GCP3_HUMAN GCP3_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP2_HUMAN GCP2_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. | | [https://www.uniprot.org/uniprot/GCP3_HUMAN GCP3_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The gamma-tubulin ring complex (gamma-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human gamma-TuRC at approximately 3.8 A resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the gamma-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the gamma-TuRC lumen. Despite its asymmetric architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the gamma-TuRC subunits introduces large (>100,000 A(2)) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the gamma-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.
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| Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex.,Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189<ref>PMID:31862189</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6v6b" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Tubulin 3D Structures|Tubulin 3D Structures]] | | *[[Tubulin 3D Structures|Tubulin 3D Structures]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chait, B T]] | | [[Category: Chait BT]] |
| [[Category: Kapoor, T M]] | | [[Category: Kapoor TM]] |
| [[Category: Molloy, K R]] | | [[Category: Molloy KR]] |
| [[Category: Ti, S]] | | [[Category: Ti S]] |
| [[Category: Urnavicius, L]] | | [[Category: Urnavicius L]] |
| [[Category: Wieczorek, M]] | | [[Category: Wieczorek M]] |
| [[Category: G-turc]]
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| [[Category: Gamma-tubulin ring complex]]
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| [[Category: Gamma-tusc]]
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| [[Category: Gcp]]
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| [[Category: Gcp2]]
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| [[Category: Gcp3]]
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| [[Category: Gturc]]
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| [[Category: Gtusc]]
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| [[Category: Microtubule]]
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| [[Category: Microtubule nucleation]]
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| [[Category: Single particle cryo-em structure]]
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| [[Category: Structural protein]]
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