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| <SX load='6e0f' size='340' side='right' viewer='molstar' caption='[[6e0f]], [[Resolution|resolution]] 3.70Å' scene=''> | | <SX load='6e0f' size='340' side='right' viewer='molstar' caption='[[6e0f]], [[Resolution|resolution]] 3.70Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6e0f]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6E0F FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6e0f]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E0F FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6e0g|6e0g]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mTXNPx, LINJ_23_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5671 LEIIN])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0f OCA], [https://pdbe.org/6e0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e0f RCSB], [https://www.ebi.ac.uk/pdbsum/6e0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0f ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0f OCA], [http://pdbe.org/6e0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0f RCSB], [http://www.ebi.ac.uk/pdbsum/6e0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0f ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/A0A6L0XFC6_LEIIN A0A6L0XFC6_LEIIN] Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239] |
| Many 2-Cys-peroxiredoxins (2-Cys-Prxs) are dual-function proteins, either acting as peroxidases under non-stress conditions or as chaperones during stress. The mechanism by which 2-Cys-Prxs switch functions remains to be defined. Our work focuses on Leishmania infantum mitochondrial 2-Cys-Prx, whose reduced, decameric subpopulation adopts chaperone function during heat shock, an activity that facilitates the transition from insects to warm-blooded host environments. Here, we have solved the cryo-EM structure of mTXNPx in complex with a thermally unfolded client protein, and revealed that the flexible N-termini of mTXNPx form a well-resolved central belt that contacts and encapsulates the unstructured client protein in the center of the decamer ring. In vivo and in vitro cross-linking studies provide further support for these interactions, and demonstrate that mTXNPx decamers undergo temperature-dependent structural rearrangements specifically at the dimer-dimer interfaces. These structural changes appear crucial for exposing chaperone-client binding sites that are buried in the peroxidase-active protein.
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| Chaperone activation and client binding of a 2-cysteine peroxiredoxin.,Teixeira F, Tse E, Castro H, Makepeace KAT, Meinen BA, Borchers CH, Poole LB, Bardwell JC, Tomas AM, Southworth DR, Jakob U Nat Commun. 2019 Feb 8;10(1):659. doi: 10.1038/s41467-019-08565-8. PMID:30737390<ref>PMID:30737390</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6e0f" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]] | | *[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Leiin]] | | [[Category: Leishmania infantum]] |
| [[Category: Borchers, C H]] | | [[Category: Borchers CH]] |
| [[Category: Castro, H]] | | [[Category: Castro H]] |
| [[Category: Jakob, U]] | | [[Category: Jakob U]] |
| [[Category: Makepeace, K A.T]] | | [[Category: Makepeace KAT]] |
| [[Category: Poole, L B]] | | [[Category: Poole LB]] |
| [[Category: Southworth, D R]] | | [[Category: Southworth DR]] |
| [[Category: Teixeira, F]] | | [[Category: Teixeira F]] |
| [[Category: Tomas, A M]] | | [[Category: Tomas AM]] |
| [[Category: Tse, E]] | | [[Category: Tse E]] |
| [[Category: Chaperone]]
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| [[Category: Client-binding]]
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| [[Category: Heat-shock]]
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| [[Category: Holdase]]
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| [[Category: Unfolding]]
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