2x7n: Difference between revisions

Latest revision as of 13:21, 9 May 2024

Mechanism of eIF6s anti-association activityMechanism of eIF6s anti-association activity

2x7n, resolution 11.80Å

Structural highlights

2x7n is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 11.8Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL23A_YEAST

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.

Mechanism of eIF6-mediated inhibition of ribosomal subunit joining.,Gartmann M, Blau M, Armache JP, Mielke T, Topf M, Beckmann R J Biol Chem. 2010 May 14;285(20):14848-51. Epub 2010 Mar 31. PMID:20356839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gartmann M, Blau M, Armache JP, Mielke T, Topf M, Beckmann R. Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem. 2010 May 14;285(20):14848-51. Epub 2010 Mar 31. PMID:20356839 doi:10.1074/jbc.C109.096057

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OCA

[1] Gartmann M, Blau M, Armache JP, Mielke T, Topf M, Beckmann R. Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem. 2010 May 14;285(20):14848-51. Epub 2010 Mar 31. PMID:20356839 doi:10.1074/jbc.C109.096057

[1]

@@ Line 3: / Line 3: @@
 <SX load='2x7n' size='340' side='right' viewer='molstar' caption='[[2x7n]], [[Resolution|resolution]] 11.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x7n]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2X7N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x7n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X7N FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1k5y|1k5y]], [[1g62|1g62]], [[1s1i|1s1i]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2x7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7n OCA], [http://pdbe.org/2x7n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x7n RCSB], [http://www.ebi.ac.uk/pdbsum/2x7n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x7n ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7n OCA], [https://pdbe.org/2x7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x7n RCSB], [https://www.ebi.ac.uk/pdbsum/2x7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x7n ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IF6_YEAST IF6_YEAST]] Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by the GTPase RIA1/EFL1 and by SDO1. Also required for pre-rRNA processing.<ref>PMID:10085284</ref> <ref>PMID:11779510</ref> <ref>PMID:11238882</ref> <ref>PMID:17353896</ref> <ref>PMID:18256024</ref> <ref>PMID:11101899</ref>
+[https://www.uniprot.org/uniprot/RL23A_YEAST RL23A_YEAST]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 39: / Line 39: @@
 [[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Armache, J P]]
+[[Category: Armache J-P]]
-[[Category: Beckmann, R]]
+[[Category: Beckmann R]]
-[[Category: Blau, M]]
+[[Category: Blau M]]
-[[Category: Gartmann, M]]
+[[Category: Gartmann M]]
-[[Category: Mielke, T]]
+[[Category: Mielke T]]
-[[Category: Topf, M]]
+[[Category: Topf M]]
-[[Category: Initiation factor]]
-[[Category: Protein biosynthesis]]
-[[Category: Ribonucleoprotein]]
-[[Category: Ribosomal protein]]
-[[Category: Ribosomal protein-rna complex]]

2x7n: Difference between revisions

Latest revision as of 13:21, 9 May 2024

Mechanism of eIF6s anti-association activityMechanism of eIF6s anti-association activity

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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2x7n: Difference between revisions

Latest revision as of 13:21, 9 May 2024

Mechanism of eIF6s anti-association activityMechanism of eIF6s anti-association activity

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

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