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| [[Image:1awd.gif|left|200px]]
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| <!-- | | ==FERREDOXIN [2FE-2S] OXIDIZED FORM FROM CHLORELLA FUSCA== |
| The line below this paragraph, containing "STRUCTURE_1awd", creates the "Structure Box" on the page.
| | <StructureSection load='1awd' size='340' side='right'caption='[[1awd]], [[Resolution|resolution]] 1.40Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1awd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorella_fusca Chlorella fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AWD FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
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| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> |
| {{STRUCTURE_1awd| PDB=1awd | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1awd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1awd OCA], [https://pdbe.org/1awd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1awd RCSB], [https://www.ebi.ac.uk/pdbsum/1awd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1awd ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/FER_SCEFU FER_SCEFU] |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/1awd_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1awd ConSurf]. |
| | <div style="clear:both"></div> |
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| '''FERREDOXIN [2FE-2S] OXIDIZED FORM FROM CHLORELLA FUSCA'''
| | ==See Also== |
| | | *[[Ferredoxin 3D structures|Ferredoxin 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| BACKGROUND: [2Fe-2S] ferredoxins, also called plant-type ferredoxins, are low-potential redox proteins that are widely distributed in biological systems. In photosynthesis, the plant-type ferredoxins function as the central molecule for distributing electrons from the photolysis of water to a number of ferredox-independent enzymes, as well as to cyclic photophosphorylation electron transfer. This paper reports only the second structure of a [2Fe-2S] ferredoxin from a eukaryotic organism in its native form. RESULTS: Ferredoxin from the green algae Chlorella fusca has been purified, characterised, crystallised and its structure determined to 1.4 A resolution - the highest resolution structure published to date for a plant-type ferredoxin. The structure has the general features of the plant-type ferredoxins already described, with conformational differences corresponding to regions of higher mobility. Immunological data indicate that a serine residue within the protein is partially phosphorylated. A slightly electropositive shift in the measured redox potential value, -325 mV, is observed in comparison with other ferredoxins. CONCLUSIONS: This high-resolution structure provides a detailed picture of the hydrogen-bonding pattern around the [2Fe-2S] cluster of a plant-type ferredoxin; for the first time, it was possible to obtain reliable error estimates for the geometrical parameters. The presence of phosphoserine in the protein indicates a possible mechanism for the regulation of the distribution of reducing power from the photosynthetic electron-transfer chain.
| | [[Category: Large Structures]] |
| | | [[Category: Sheldrick GM]] |
| ==About this Structure==
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| 1AWD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWD OCA].
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| ==Reference==
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| Crystal structure determination at 1.4 A resolution of ferredoxin from the green alga Chlorella fusca., Bes MT, Parisini E, Inda LA, Saraiva LM, Peleato ML, Sheldrick GM, Structure. 1999 Oct 15;7(10):1201-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10545324 10545324]
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| [[Category: Eukaryota]] | |
| [[Category: Single protein]]
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| [[Category: Sheldrick, G M.]] | |
| [[Category: Electron transfer]]
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| [[Category: Electron transport]]
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| [[Category: Eukaryotic]]
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| [[Category: Green alga]]
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| [[Category: Metalloprotein]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:46:32 2008''
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