5aey

actin-like ParM protein bound to AMPPNPactin-like ParM protein bound to AMPPNP

5aey, resolution 4.30Å Show:Asymmetric Unit Biological Assembly Structural highlights 5aey is a 5 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.3Å Ligands: Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PARM_ECOLX Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid. Publication Abstract from PubMed Active segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 A resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.,Bharat TA, Murshudov GN, Sachse C, Lowe J Nature. 2015 Apr 27:1-5. doi: 10.1038/nature14356. PMID:25915019 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Plasmid segregation protein ParM