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| <SX load='3jav' size='340' side='right' viewer='molstar' caption='[[3jav]], [[Resolution|resolution]] 4.70Å' scene=''> | | <SX load='3jav' size='340' side='right' viewer='molstar' caption='[[3jav]], [[Resolution|resolution]] 4.70Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3jav]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAV FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3jav]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAV FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jav OCA], [http://pdbe.org/3jav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jav RCSB], [http://www.ebi.ac.uk/pdbsum/3jav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jav ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jav OCA], [https://pdbe.org/3jav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jav RCSB], [https://www.ebi.ac.uk/pdbsum/3jav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jav ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/ITPR1_RAT ITPR1_RAT]] Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity). | | [https://www.uniprot.org/uniprot/ITPR1_RAT ITPR1_RAT] Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity). |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Inositol-1,4,5-trisphosphate receptors (InsP3Rs) are ubiquitous ion channels responsible for cytosolic Ca2+ signalling and essential for a broad array of cellular processes ranging from contraction to secretion, and from proliferation to cell death. Despite decades of research on InsP3Rs, a mechanistic understanding of their structure-function relationship is lacking. Here we present the first, to our knowledge, near-atomic (4.7 A) resolution electron cryomicroscopy structure of the tetrameric mammalian type 1 InsP3R channel in its apo-state. At this resolution, we are able to trace unambiguously approximately 85% of the protein backbone, allowing us to identify the structural elements involved in gating and modulation of this 1.3-megadalton channel. Although the central Ca2+-conduction pathway is similar to other ion channels, including the closely related ryanodine receptor, the cytosolic carboxy termini are uniquely arranged in a left-handed alpha-helical bundle, directly interacting with the amino-terminal domains of adjacent subunits. This configuration suggests a molecular mechanism for allosteric regulation of channel gating by intracellular signals.
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| Gating machinery of InsPR channels revealed by electron cryomicroscopy.,Fan G, Baker ML, Wang Z, Baker MR, Sinyagovskiy PA, Chiu W, Ludtke SJ, Serysheva II Nature. 2015 Oct 12. doi: 10.1038/nature15249. PMID:26458101<ref>PMID:26458101</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3jav" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Inositol 1%2C4%2C5-Trisphosphate Receptor|Inositol 1%2C4%2C5-Trisphosphate Receptor]] | | *[[Inositol 1%2C4%2C5-Trisphosphate Receptor|Inositol 1%2C4%2C5-Trisphosphate Receptor]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Rattus norvegicus]] | | [[Category: Rattus norvegicus]] |
| [[Category: Baker, M L]] | | [[Category: Baker ML]] |
| [[Category: Baker, M R]] | | [[Category: Baker MR]] |
| [[Category: Chiu, W]] | | [[Category: Chiu W]] |
| [[Category: Fan, G]] | | [[Category: Fan G]] |
| [[Category: Ludtke, S J]] | | [[Category: Ludtke SJ]] |
| [[Category: Serysheva, I I]] | | [[Category: Serysheva II]] |
| [[Category: Sinyagovskiy, P A]] | | [[Category: Sinyagovskiy PA]] |
| [[Category: Wang, Z]] | | [[Category: Wang Z]] |
| [[Category: 5-trisphosphate receptor]]
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| [[Category: Calcium release channel]]
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| [[Category: Calcium signaling]]
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| [[Category: Inositol 1]]
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| [[Category: Transport protein]]
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