Transposase: Difference between revisions
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<StructureSection load='3ecp' size='350' side='right' caption='Structure of Tn5 transposase complex with DNA and glycerol (PDB code [[3ecp]]).' scene=''> | <StructureSection load='3ecp' size='350' side='right' caption='Structure of Tn5 transposase complex with DNA and glycerol (PDB code [[3ecp]]).' scene='59/596996/Cv/8'> | ||
__TOC__ | __TOC__ | ||
== Function == | == Function == | ||
'''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. <br /> | '''Transposases''' or '''transposition proteins''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. <br /> | ||
*'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> | *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> | ||
*'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> | *'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> | ||
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== Structural highlights == | == Structural highlights == | ||
Transposase contains a <scene name='59/596996/Cv/ | <scene name='59/596996/Cv/9'>The Biological Assembly of Tn5 transposase is homodimer</scene> (PDB code [[3ecp]]). Transposase contains a <scene name='59/596996/Cv/7'>DDE motif in its active site</scene><ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity. | ||
==3D structures of transposase== | ==3D structures of transposase== | ||
[[Transposase 3D structures]] | [[Transposase 3D structures]] | ||