6l7h: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='6l7h' size='340' side='right'caption='[[6l7h]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='6l7h' size='340' side='right'caption='[[6l7h]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6l7h]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6l7h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycyrrhiza_glabra Glycyrrhiza glabra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L7H FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E7F:1-[3-[(2S,3R,4R,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]-2,4,6-tris(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one'>E7F</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E7F:1-[3-[(2S,3R,4R,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]-2,4,6-tris(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one'>E7F</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l7h OCA], [https://pdbe.org/6l7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l7h RCSB], [https://www.ebi.ac.uk/pdbsum/6l7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l7h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 16: | Line 17: | ||
</div> | </div> | ||
<div class="pdbe-citations 6l7h" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6l7h" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Glycyrrhiza glabra]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Li | [[Category: Li FD]] | ||
[[Category: Ye | [[Category: Ye M]] | ||
[[Category: Zhang | [[Category: Zhang M]] | ||
Latest revision as of 13:52, 22 November 2023
crystal structure of GgCGT in complex with UDP and Nothofagincrystal structure of GgCGT in complex with UDP and Nothofagin
Structural highlights
Publication Abstract from PubMedA highly efficient di-C-glycosyltransferase GgCGT was discovered from the medicinal plant Glycyrrhiza glabra. GgCGT catalyzes a two-step di-C-glycosylation of flopropione-containing substrates with conversion rates of >98%. To elucidate the catalytic mechanisms of GgCGT, we solved its crystal structures in complex with UDP-Glc, UDP-Gal, UDP/phloretin, and UDP/nothofagin, respectively. Structural analysis revealed that the sugar donor selectivity was controlled by the hydrogen-bond interactions of sugar hydroxyl groups with D390 and other key residues. The di-C-glycosylation capability of GgCGT was attributed to a spacious substrate-binding tunnel, and the G389K mutation could switch di- to mono-C-glycosylation. GgCGT is the first di-C-glycosyltransferase with a crystal structure, and the first C-glycosyltransferase with a complex structure containing a sugar acceptor. This work could benefit the development of efficient biocatalysts to synthesize C-glycosides with medicinal potential. Functional Characterization and Structural Basis of an Efficient Di-C-glycosyltransferase from Glycyrrhiza glabra.,Zhang M, Li FD, Li K, Wang ZL, Wang YX, He JB, Su HF, Zhang ZY, Chi CB, Shi XM, Yun CH, Zhang ZY, Liu ZM, Zhang LR, Yang DH, Ma M, Qiao X, Ye M J Am Chem Soc. 2020 Feb 11. doi: 10.1021/jacs.9b12211. PMID:31986016[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||