5r4r: Difference between revisions

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New page: '''Unreleased structure''' The entry 5r4r is ON HOLD Authors: Kidd, S.L., Mateu, N., Talon, R., Krojer, T., Aimon, A., Bradley, A.R., Fairhead, M., Díaz...
 
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'''Unreleased structure'''


The entry 5r4r is ON HOLD
==PanDDA analysis group deposition -- Crystal Structure of HUMAN CLEAVAGE FACTOR IM in complex with EN08775-42==
 
<StructureSection load='5r4r' size='340' side='right'caption='[[5r4r]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
Authors: Kidd, S.L., Mateu, N., Talon, R., Krojer, T., Aimon, A., Bradley, A.R., Fairhead, M., Díaz-Sáez, L., Sore, H.F., Madin, A., Huber, K.V.M., von Delft, F., Spring, D.R.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[5r4r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5R4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5R4R FirstGlance]. <br>
Description: PanDDA analysis group deposition --Crystal Structure of HUMAN CLEAVAGE FACTOR IM in complex with EN08775-42
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=RWA:(5R)-3,3,5-trimethyl-5-[(1-phenyl-1H-1,2,3-triazol-4-yl)methyl]pyrrolidine-2,4-dione'>RWA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
[[Category: Spring, D.R]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5r4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5r4r OCA], [https://pdbe.org/5r4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5r4r RCSB], [https://www.ebi.ac.uk/pdbsum/5r4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5r4r ProSAT]</span></td></tr>
[[Category: DãƒÆ’ƒÀšÃ‚­Az-SãƒÆ’ƒÀšÃ‚¡Ez, L]]
</table>
[[Category: Aimon, A]]
== Function ==
[[Category: Mateu, N]]
[https://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref>
[[Category: Kidd, S.L]]
== References ==
[[Category: Madin, A]]
<references/>
[[Category: Fairhead, M]]
__TOC__
[[Category: Von Delft, F]]
</StructureSection>
[[Category: Bradley, A.R]]
[[Category: Homo sapiens]]
[[Category: Talon, R]]
[[Category: Large Structures]]
[[Category: Huber, K.V.M]]
[[Category: Aimon A]]
[[Category: Sore, H.F]]
[[Category: Bradley AR]]
[[Category: Krojer, T]]
[[Category: Diaz-Saez L]]
[[Category: Fairhead M]]
[[Category: Huber KVM]]
[[Category: Kidd SL]]
[[Category: Krojer T]]
[[Category: Madin A]]
[[Category: Mateu N]]
[[Category: Sore HF]]
[[Category: Spring DR]]
[[Category: Talon R]]
[[Category: Von Delft F]]

Latest revision as of 14:35, 21 February 2024

PanDDA analysis group deposition -- Crystal Structure of HUMAN CLEAVAGE FACTOR IM in complex with EN08775-42PanDDA analysis group deposition -- Crystal Structure of HUMAN CLEAVAGE FACTOR IM in complex with EN08775-42

Structural highlights

5r4r is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPSF5_HUMAN Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.[1] [2] [3] [4] [5] [6]

References

  1. ↑ Ruegsegger U, Blank D, Keller W. Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Mol Cell. 1998 Jan;1(2):243-53. PMID:9659921
  2. ↑ Ruegsegger U, Beyer K, Keller W. Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors. J Biol Chem. 1996 Mar 15;271(11):6107-13. PMID:8626397
  3. ↑ Brown KM, Gilmartin GM. A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im. Mol Cell. 2003 Dec;12(6):1467-76. PMID:14690600
  4. ↑ Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, Yamaguchi Y. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells. 2010 Sep 1;15(9):1003-13. doi: 10.1111/j.1365-2443.2010.01436.x., Epub 2010 Jul 29. PMID:20695905 doi:10.1111/j.1365-2443.2010.01436.x
  5. ↑ Yang Q, Gilmartin GM, Doublie S. Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10062-7. Epub 2010 May 17. PMID:20479262
  6. ↑ Yang Q, Coseno M, Gilmartin GM, Doublie S. Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping. Structure. 2011 Feb 2. PMID:21295486 doi:10.1016/j.str.2010.12.021

5r4r, resolution 1.50Å

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