2bn5: Difference between revisions
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==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide== | ==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide== | ||
<StructureSection load='2bn5' size='340' side='right'caption='[[2bn5 | <StructureSection load='2bn5' size='340' side='right'caption='[[2bn5]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BN5 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn5 OCA], [https://pdbe.org/2bn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bn5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q7JPS0_DROSP Q7JPS0_DROSP] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Butler | [[Category: Butler PJG]] | ||
[[Category: Ignjatovic | [[Category: Ignjatovic T]] | ||
[[Category: Nagai | [[Category: Nagai K]] | ||
[[Category: Neuhaus | [[Category: Neuhaus D]] | ||
[[Category: Yang | [[Category: Yang J-C]] | ||
Latest revision as of 16:35, 30 August 2023
P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptideP-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide
Structural highlights
FunctionPublication Abstract from PubMedP-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein. Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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