6lr3: Difference between revisions
New page: '''Unreleased structure''' The entry 6lr3 is ON HOLD Authors: Su, Z.M., Tian, X.Y., Li, H.J., Wei, Z.M., Chen, L.F., Ren, H.X., Peng, W.F., Tang, C.T. Description: Structural and funct... |
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==Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum== | |||
<StructureSection load='6lr3' size='340' side='right'caption='[[6lr3]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6lr3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncomelania_hupensis Oncomelania hupensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LR3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lr3 OCA], [https://pdbe.org/6lr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lr3 RCSB], [https://www.ebi.ac.uk/pdbsum/6lr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lr3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A1U9W5E8_9CAEN A0A1U9W5E8_9CAEN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum. As an irreplaceable prerequisite in the transmission and prevalence of schistosomiasis japonica, an in-depth study of this obligate host-parasite interaction can provide glimpse into the molecular events in the competition between schistosome infectivity and snail immune resistance. In previous studies, we identified a macrophage migration inhibitory factor (MIF) from O. hupensis (OhMIF), and showed that it was involved in the snail host immune response to the parasite S. japonicum. Here, we determined the crystal structure of OhMIF and revealed that there were distinct structural differences between the mammalian and O. hupensis MIFs. Noticeably, there was a projecting and structured C-terminus in OhMIF, which not only regulated the MIF's thermostability but was also critical in the activation of its tautomerase activity. Comparative studies between OhMIF and human MIF (hMIF) by analyzing the tautomerase activity, oxidoreductase activity, thermostability, interaction with the receptor CD74 and activation of the ERK signaling pathway demonstrated the functional differences between hMIF and OhMIF. Our data shed a species-specific light on structural, functional, and immunological characteristics of OhMIF and enrich the knowledge on the MIF family. | |||
Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum.,Su Z, Tian X, Li H, Wei Z, Chen L, Wang S, Ren H, Peng W, Tang C, Lin T, Huang S Biochem J. 2020 Jun 26;477(12):2133-2151. doi: 10.1042/BCJ20200068. PMID:32484230<ref>PMID:32484230</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6lr3" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Macrophage inhibitory factor 3D structures|Macrophage inhibitory factor 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Su | <references/> | ||
[[Category: Tian | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Oncomelania hupensis]] | |||
[[Category: Chen LF]] | |||
[[Category: Li HJ]] | |||
[[Category: Peng WF]] | |||
[[Category: Ren HX]] | |||
[[Category: Su ZM]] | |||
[[Category: Tang CT]] | |||
[[Category: Tian XY]] | |||
[[Category: Wei ZM]] | |||
Latest revision as of 17:45, 29 November 2023
Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicumStructural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum
Structural highlights
FunctionPublication Abstract from PubMedOncomelania hupensis is the unique intermediate host of Schistosoma japonicum. As an irreplaceable prerequisite in the transmission and prevalence of schistosomiasis japonica, an in-depth study of this obligate host-parasite interaction can provide glimpse into the molecular events in the competition between schistosome infectivity and snail immune resistance. In previous studies, we identified a macrophage migration inhibitory factor (MIF) from O. hupensis (OhMIF), and showed that it was involved in the snail host immune response to the parasite S. japonicum. Here, we determined the crystal structure of OhMIF and revealed that there were distinct structural differences between the mammalian and O. hupensis MIFs. Noticeably, there was a projecting and structured C-terminus in OhMIF, which not only regulated the MIF's thermostability but was also critical in the activation of its tautomerase activity. Comparative studies between OhMIF and human MIF (hMIF) by analyzing the tautomerase activity, oxidoreductase activity, thermostability, interaction with the receptor CD74 and activation of the ERK signaling pathway demonstrated the functional differences between hMIF and OhMIF. Our data shed a species-specific light on structural, functional, and immunological characteristics of OhMIF and enrich the knowledge on the MIF family. Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum.,Su Z, Tian X, Li H, Wei Z, Chen L, Wang S, Ren H, Peng W, Tang C, Lin T, Huang S Biochem J. 2020 Jun 26;477(12):2133-2151. doi: 10.1042/BCJ20200068. PMID:32484230[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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