Reverse transcriptase: Difference between revisions
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==Introduction== | ==Introduction== | ||
[[Reverse transcriptase]] (RT) or '''RNA-dependent DNA polymerase''' transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51 subchains. P15 is its RNAse H domain. There are two types of inhibitors for RT: NNRTIs are the non-nucleoside inhibitors, and NRTIs are the | [[Reverse transcriptase]] (RT) or '''RNA-dependent DNA polymerase''' transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51 subchains. P15 is its RNAse H domain. There are two types of inhibitors for RT: '''NNRTIs''' are the non-nucleoside inhibitors, and '''NRTIs''' are the nucleoside inhibitors. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, along with [[Hiv protease|Protease]] and Integrase, the most important part of the protein system involved in the process of infection and reproduction for viruses like HIV, MuLV and AMV. RT has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology. | ||
Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries. See also <br /> | Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries. See also <br /> | ||
*[[Transcription and RNA Processing]]<br /> | *[[Transcription and RNA Processing]]<br /> | ||
*[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br /> | *[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br /> | ||
*[[Efavirenz]]<br /> | |||
*[[Emtricitabine]]<br /> | |||
*[[Efavirenz/emtricitabine/tenofovir]]<br /> | |||
*[[Phl p 2]]<br /> | *[[Phl p 2]]<br /> | ||
*[[Tenofovir disoproxil]]<br /> | |||
*[[AZT-resistant HIV-1 reverse transcriptase]]<br /> | *[[AZT-resistant HIV-1 reverse transcriptase]]<br /> | ||
*[[Catalytic Subunit of T. Castaneum TERT Polymerase]].<br /> | *[[Catalytic Subunit of T. Castaneum TERT Polymerase]].<br /> | ||