5e9t: Difference between revisions

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 <StructureSection load='5e9t' size='340' side='right'caption='[[5e9t]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5e9t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E9T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E9T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5e9t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E9T FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5e9u|5e9u]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e9t OCA], [https://pdbe.org/5e9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e9t RCSB], [https://www.ebi.ac.uk/pdbsum/5e9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e9t ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtf1, gtfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558]), gtf2, gtfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e9t OCA], [http://pdbe.org/5e9t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e9t RCSB], [http://www.ebi.ac.uk/pdbsum/5e9t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e9t ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GTF1_STRGN GTF1_STRGN]] An N-acetylglucosaminyl transferase that is part of the accessory SecA2/SecY2 system specifically required to export GspB, a serine-rich repeat cell wall protein usually encoded upstream in the same operon. Required for correct glycosylation of GspB. Upon coexpression in E.coli with Gtf2 (GtfB) glycosylates GspB constructs. Glycosylation probably occurs intracellularly.<ref>PMID:15489421</ref>  [[http://www.uniprot.org/uniprot/GTF2_STRGN GTF2_STRGN]] A stabilizing protein that is part of the accessory SecA2/SecY2 system specifically required to export GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon. Required for correct glycosylation of GspB. Upon coexpression in E.coli with Gtf1 (GtfA) glycosylates GspB constructs. Stabilizes the glycosylation activity of Gtf1 (By similarity).<ref>PMID:15489421</ref>
+[https://www.uniprot.org/uniprot/GTFA_STRGN GTFA_STRGN] Required for polymorphic O-glycosylation of GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in GspB. Part of the accessory SecA2/SecY2 system specifically required to export GspB. Upon coexpression in E.coli with GtfB glycosylates GspB constructs. Glycosylation probably occurs intracellularly (PubMed:15489421). Requires GtfB for glycosylation activity, it has no activity alone. Does not use UDP-glucose as substrate. Has a fast, probably processive glycosylation phase followed by a slower, non-processive phase. The enzyme probably modifies its tertiary conformation by opening and closing its intersubunit interfaces to accomodate the increasingly glycosylated substrate; protein substrate recognition is provided by GtfB (PubMed:26884191).<ref>PMID:15489421</ref> <ref>PMID:26884191</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
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 __TOC__
 </StructureSection>
-[[Category: Atcc 10558]]
 [[Category: Large Structures]]
-[[Category: Chen, Y]]
+[[Category: Streptococcus gordonii]]
-[[Category: Rapoport, T A]]
+[[Category: Chen Y]]
-[[Category: Accessory protein translocation]]
+[[Category: Rapoport TA]]
-[[Category: Complex]]
-[[Category: Glycosyltransferase]]
-[[Category: Transferase-chaperone complex]]