6nie: Difference between revisions

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<StructureSection load='6nie' size='340' side='right'caption='[[6nie]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='6nie' size='340' side='right'caption='[[6nie]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6nie]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Stren Stren]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NIE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NIE FirstGlance]. <br>
<table><tr><td colspan='2'>[[6nie]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya_NRRL_8057_=_DSM_46488 Streptomyces cattleya NRRL 8057 = DSM 46488]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NIE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCATT_p06880 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1003195 STREN])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nie OCA], [http://pdbe.org/6nie PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nie RCSB], [http://www.ebi.ac.uk/pdbsum/6nie PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nie ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nie OCA], [https://pdbe.org/6nie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nie RCSB], [https://www.ebi.ac.uk/pdbsum/6nie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nie ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/BESD_STREN BESD_STREN]] Involved in the biosynthesis of terminal alkyne-containing amino acids produced as antibiotics by S.cattleya. Catalyzes the chlorination of L-lysine at the C4 position, leading to the production of 4-chloro-L-lysine.<ref>PMID:30867596</ref> 
[https://www.uniprot.org/uniprot/BESD_STREN BESD_STREN] Involved in the biosynthesis of terminal alkyne-containing amino acids produced as antibiotics by S.cattleya. Catalyzes the chlorination of L-lysine at the C4 position, leading to the production of 4-chloro-L-lysine.<ref>PMID:30867596</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The integration of synthetic and biological catalysis enables new approaches to the synthesis of small molecules by combining the high selectivity of enzymes with the reaction diversity offered by synthetic chemistry. While organohalogens are valued for their bioactivity and utility as synthetic building blocks, only a handful of enzymes that carry out the regioselective halogenation of unactivated [Formula: see text] bonds have previously been identified. In this context, we report the structural characterization of BesD, a recently discovered radical halogenase from the Fe(II)/alpha-ketogluturate-dependent family that chlorinates the free amino acid lysine. We also identify and characterize additional halogenases that produce mono- and dichlorinated, as well as brominated and azidated, amino acids. The substrate selectivity of this new family of radical halogenases takes advantage of the central role of amino acids in metabolism and enables engineering of biosynthetic pathways to afford a wide variety of compound classes, including heterocycles, diamines, alpha-keto acids and peptides.
 
A family of radical halogenases for the engineering of amino-acid-based products.,Neugebauer ME, Sumida KH, Pelton JG, McMurry JL, Marchand JA, Chang MCY Nat Chem Biol. 2019 Oct;15(10):1009-1016. doi: 10.1038/s41589-019-0355-x. Epub, 2019 Sep 23. PMID:31548692<ref>PMID:31548692</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6nie" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Stren]]
[[Category: Streptomyces cattleya NRRL 8057 = DSM 46488]]
[[Category: Chang, M C.Y]]
[[Category: Chang MCY]]
[[Category: Neugebauer, M E]]
[[Category: Neugebauer ME]]
[[Category: Biosynthetic protein]]
[[Category: Halogenase]]

Latest revision as of 17:47, 13 March 2024

BesD with Fe(II), chloride, and alpha-ketoglutarateBesD with Fe(II), chloride, and alpha-ketoglutarate

Structural highlights

6nie is a 4 chain structure with sequence from Streptomyces cattleya NRRL 8057 = DSM 46488. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.95Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BESD_STREN Involved in the biosynthesis of terminal alkyne-containing amino acids produced as antibiotics by S.cattleya. Catalyzes the chlorination of L-lysine at the C4 position, leading to the production of 4-chloro-L-lysine.[1]

References

  1. ↑ Marchand JA, Neugebauer ME, Ing MC, Lin CI, Pelton JG, Chang MCY. Discovery of a pathway for terminal-alkyne amino acid biosynthesis. Nature. 2019 Mar;567(7748):420-424. doi: 10.1038/s41586-019-1020-y. Epub 2019 Mar, 13. PMID:30867596 doi:http://dx.doi.org/10.1038/s41586-019-1020-y

6nie, resolution 1.95Å

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