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<StructureSection load='6jp6' size='340' side='right'caption='[[6jp6]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='6jp6' size='340' side='right'caption='[[6jp6]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6jp6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JP6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JP6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6jp6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JP6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JP6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.699&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_(cytidine(32)/guanosine(34)-2'-O)-methyltransferase tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.205 2.1.1.205] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jp6 OCA], [http://pdbe.org/6jp6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jp6 RCSB], [http://www.ebi.ac.uk/pdbsum/6jp6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jp6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jp6 OCA], [https://pdbe.org/6jp6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jp6 RCSB], [https://www.ebi.ac.uk/pdbsum/6jp6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jp6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/WDR6_YEAST WDR6_YEAST]] Involved in regulation of Ty1 transposition. Plays also a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Required together with TRM7 for the methylation of the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of tRNA(Phe) and tRNA(Leu(UAA)).<ref>PMID:18202368</ref> <ref>PMID:21880895</ref> <ref>PMID:22912484</ref> [[http://www.uniprot.org/uniprot/TRM7_YEAST TRM7_YEAST]] Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of tRNA(Phe), tRNA(Trp) and tRNA(Leu(UAA)). Requires TRM732 for methylation of the cytidine at position 32 and RTT10/TRM734 for methylation of the nucleotides at position 34 in substrate tRNAs. Lack of either of these modifications in tRNA(Phe) reduces formation of wybutosine from 1-methylguanosine at position 37.<ref>PMID:11927565</ref> <ref>PMID:22912484</ref> 
[https://www.uniprot.org/uniprot/WDR6_YEAST WDR6_YEAST] Involved in regulation of Ty1 transposition. Plays also a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Required together with TRM7 for the methylation of the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of tRNA(Phe) and tRNA(Leu(UAA)).<ref>PMID:18202368</ref> <ref>PMID:21880895</ref> <ref>PMID:22912484</ref>  
 
==See Also==
*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Hirata, A]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Hori, H]]
[[Category: Hirata A]]
[[Category: Okada, K]]
[[Category: Hori H]]
[[Category: Saijo, S]]
[[Category: Okada K]]
[[Category: Shiraisi, H]]
[[Category: Saijo S]]
[[Category: Sihimzu, N]]
[[Category: Shiraisi H]]
[[Category: Yonezawa, K]]
[[Category: Sihimzu N]]
[[Category: Yoshii, K]]
[[Category: Yonezawa K]]
[[Category: Transferase]]
[[Category: Yoshii K]]
[[Category: Trna maturation]]
[[Category: Trna methyltransferase]]

Latest revision as of 13:39, 27 March 2024

The X-ray structure of yeast tRNA methyltransferase complex of Trm7 and Trm734 essential for 2'-O-methylation at the first position of anticodon in specific tRNAsThe X-ray structure of yeast tRNA methyltransferase complex of Trm7 and Trm734 essential for 2'-O-methylation at the first position of anticodon in specific tRNAs

Structural highlights

6jp6 is a 4 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.699Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

WDR6_YEAST Involved in regulation of Ty1 transposition. Plays also a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Required together with TRM7 for the methylation of the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of tRNA(Phe) and tRNA(Leu(UAA)).[1] [2] [3]

See Also

References

  1. ↑ Nyswaner KM, Checkley MA, Yi M, Stephens RM, Garfinkel DJ. Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis. Genetics. 2008 Jan;178(1):197-214. doi: 10.1534/genetics.107.082602. PMID:18202368 doi:http://dx.doi.org/10.1534/genetics.107.082602
  2. ↑ Shi Y, Stefan CJ, Rue SM, Teis D, Emr SD. Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell. 2011 Nov;22(21):4093-107. doi: 10.1091/mbc.E11-05-0440. Epub 2011 , Aug 31. PMID:21880895 doi:http://dx.doi.org/10.1091/mbc.E11-05-0440
  3. ↑ Guy MP, Podyma BM, Preston MA, Shaheen HH, Krivos KL, Limbach PA, Hopper AK, Phizicky EM. Yeast Trm7 interacts with distinct proteins for critical modifications of the tRNAPhe anticodon loop. RNA. 2012 Oct;18(10):1921-33. doi: 10.1261/rna.035287.112. Epub 2012 Aug 21. PMID:22912484 doi:http://dx.doi.org/10.1261/rna.035287.112

6jp6, resolution 2.70Å

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