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| ==Mouse norovirus complexed with GCDCA== | | ==Mouse norovirus complexed with GCDCA== |
| <StructureSection load='6p4j' size='340' side='right'caption='[[6p4j]], [[Resolution|resolution]] 3.10Å' scene=''> | | <SX load='6p4j' size='340' side='right' viewer='molstar' caption='[[6p4j]], [[Resolution|resolution]] 3.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6p4j]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P4J FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6p4j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P4J FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHO:GLYCOCHENODEOXYCHOLIC+ACID'>CHO</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p4j OCA], [http://pdbe.org/6p4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p4j RCSB], [http://www.ebi.ac.uk/pdbsum/6p4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p4j ProSAT]</span></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHO:GLYCOCHENODEOXYCHOLIC+ACID'>CHO</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p4j OCA], [https://pdbe.org/6p4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p4j RCSB], [https://www.ebi.ac.uk/pdbsum/6p4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p4j ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q2V8W4_9CALI Q2V8W4_9CALI] |
| Caliciviruses are single stranded RNA viruses with 180 copies of capsid protein comprising the T=3 icosahedral capsids. The main capsid feature is a pronounced protruding (P) domain dimer formed by adjacent subunits on the icosahedral surface while the shell domain forms a tight icosahedral sphere around the genome. While the P domain in the crystal structure of human Norwalk virus (genotype I.1) was tightly associated with the shell surface, the cryo-EM structures of several members of the Calicivirus family (mouse norovirus, rabbit hemorrhagic disease virus, and human norovirus genotype II.10) revealed a 'floating' P domain that hovers above the shell by nearly 10-15A in physiological buffers. Since this unusual feature is shared among, and unique to, the Calicivirus family, it suggests an important biological role. Recently, we demonstrated that bile salts enhance cell attachment to the target cell and increase the intrinsic affinity between the P domain and receptor. Presented here are the cryo-EM structures of MNV-1 in the presence of bile salts ( approximately 3A) and the receptor CD300lf ( approximately 8A). Surprisingly, bile salts cause the rotation and contraction of the P domain onto the shell surface. This both stabilizes the P domain and appears to allow for a higher degree of saturation of receptor onto the virus. Together, these results suggest that, as the virus moves into the gut and the associated high concentrations of bile, the entire capsid face undergoes a conformational change to optimize receptor avidity while the P domain itself undergoes smaller conformational changes to improve receptor affinity.IMPORTANCE Mouse norovirus and several other members of the Calicivirus family have been shown to have a highly unusual structure with the receptor binding protruding (P) domain only loosely tethered to the main capsid shell. Recent studies demonstrated that bile salts enhance the intrinsic P domain/receptor affinity and is necessary for cell attachment. Presented here are the high resolution cryo-EM structures of apo MNV, MNV/bile salt, and MNV/bile salt/receptor. Bile salts cause a 90 degrees rotation and collapse of the P domain onto the shell surface that may increase the number of available receptor binding sites. Therefore, bile salts appear to be having several effects on MNV. Bile salts shift the structural equilibrium of the P domain towards a form that binds the receptor and away from one that binds antibody. They may also cause the entire P domain to optimize receptor binding while burying a number of potential epitopes.
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| Bile salts alter the mouse norovirus capsid conformation; possible implications for cell attachment and immune evasion.,Sherman MB, Williams AN, Smith HQ, Nelson C, Wilen CB, Fremont DH, Virgin HW, Smith TJ J Virol. 2019 Jul 24. pii: JVI.00970-19. doi: 10.1128/JVI.00970-19. PMID:31341042<ref>PMID:31341042</ref>
| | ==See Also== |
| | | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6p4j" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Murine norovirus 1]] | | [[Category: Murine norovirus 1]] |
| [[Category: Smith, T J]] | | [[Category: Smith TJ]] |
| [[Category: Bile salt]]
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| [[Category: Norovirus]]
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| [[Category: Virus]]
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