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[[Image:2j4k.gif|left|200px]]<br />
<applet load="2j4k" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2j4k, resolution 2.20&Aring;" />
'''CRYSTAL STRUCTURE OF URIDYLATE KINASE FROM SULFOLOBUS SOLFATARICUS IN COMPLEX WITH UMP TO 2.2 ANGSTROM RESOLUTION'''<br />


==About this Structure==
==Crystal structure of uridylate kinase from Sulfolobus solfataricus in complex with UMP to 2.2 Angstrom resolution==
2J4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with CD, MG and U5P as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/UMP_kinase UMP kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.22 2.7.4.22] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4K OCA].
<StructureSection load='2j4k' size='340' side='right'caption='[[2j4k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
[[Category: Single protein]]
== Structural highlights ==
[[Category: Sulfolobus solfataricus]]
<table><tr><td colspan='2'>[[2j4k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J4K FirstGlance]. <br>
[[Category: UMP kinase]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
[[Category: Jensen, K.F.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
[[Category: Jensen, K.S.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4k OCA], [https://pdbe.org/2j4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j4k RCSB], [https://www.ebi.ac.uk/pdbsum/2j4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j4k ProSAT]</span></td></tr>
[[Category: Johansson, E.]]
</table>
[[Category: CD]]
== Function ==
[[Category: MG]]
[https://www.uniprot.org/uniprot/PYRH_SACS2 PYRH_SACS2] Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP, although much less efficiently.<ref>PMID:17297917</ref>
[[Category: U5P]]
== Evolutionary Conservation ==
[[Category: aspartokinase family]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: kinase]]
Check<jmol>
[[Category: nucleoside monophosphate kinase]]
  <jmolCheckbox>
[[Category: pyrimidine biosynthesis]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j4/2j4k_consurf.spt"</scriptWhenChecked>
[[Category: pyrimidine nucleotide synthesis]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: transferase]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: ump kinase]]
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j4k ConSurf].
<div style="clear:both"></div>


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 18:25:06 2007''
==See Also==
*[[Uridylate kinase|Uridylate kinase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Jensen KF]]
[[Category: Jensen KS]]
[[Category: Johansson E]]

Latest revision as of 09:41, 1 May 2024

Crystal structure of uridylate kinase from Sulfolobus solfataricus in complex with UMP to 2.2 Angstrom resolutionCrystal structure of uridylate kinase from Sulfolobus solfataricus in complex with UMP to 2.2 Angstrom resolution

Structural highlights

2j4k is a 6 chain structure with sequence from Saccharolobus solfataricus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYRH_SACS2 Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP, although much less efficiently.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Jensen KS, Johansson E, Jensen KF. Structural and enzymatic investigation of the Sulfolobus solfataricus uridylate kinase shows competitive UTP inhibition and the lack of GTP stimulation. Biochemistry. 2007 Mar 13;46(10):2745-57. Epub 2007 Feb 13. PMID:17297917 doi:10.1021/bi0618159

2j4k, resolution 2.20Å

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