6ovk: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 6ovk is ON HOLD
+==Crystal Structure of the Pseudomonas capeferrum Anti-sigma Regulator PupR C-terminal Cell-surface Signaling Domain in Complex with the Outer Membrane Transporter PupB N-terminal Signaling Domain==
+<StructureSection load='6ovk' size='340' side='right'caption='[[6ovk]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ovk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_capeferrum Pseudomonas capeferrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OVK FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.761&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ovk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ovk OCA], [https://pdbe.org/6ovk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ovk RCSB], [https://www.ebi.ac.uk/pdbsum/6ovk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ovk ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cell-surface signaling (CSS) in Gram-negative bacteria involves highly conserved regulatory pathways that optimize gene expression by transducing extracellular environmental signals to the cytoplasm via inner-membrane sigma regulators. The molecular details of ferric siderophore-mediated activation of the iron import machinery through a sigma regulator are unclear. Here, we present the 1.56 A resolution structure of the periplasmic complex of the C-terminal CSS domain (CCSSD) of PupR, the sigma regulator in the Pseudomonas capeferrum pseudobactin BN7/8 transport system, and the N-terminal signaling domain (NTSD) of PupB, an outer-membrane TonB-dependent transducer. The structure revealed that the CCSSD consists of two subdomains: a juxta-membrane subdomain, which has a novel all-beta fold, followed by a secretin/TonB, short N-terminal subdomain at the C-terminus of the CCSSD, a previously unobserved topological arrangement of this domain. Using affinity pull-down assays, isothermal titration calorimetry, and thermal denaturation circular dichroism spectroscopy; we show that both subdomains are required for binding the NTSD with micromolar affinity and that NTSD binding improves CCSSD stability. Our findings prompt us to present a revised model of CSS wherein the CCSSD:NTSD complex forms prior to ferric-siderophore binding. Upon siderophore binding, conformational changes in the CCSSD enable regulated intramembrane proteolysis of the sigma regulator, ultimately resulting in transcriptional regulation.
-Authors:
+Structural basis of cell surface signaling by a conserved sigma regulator in Gram-negative bacteria.,Jensen JL, Jernberg BD, Sinha S, Colbert CL J Biol Chem. 2020 Feb 26. pii: RA119.010697. doi: 10.1074/jbc.RA119.010697. PMID:32107313<ref>PMID:32107313</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6ovk" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pseudomonas capeferrum]]
+[[Category: Colbert CL]]
+[[Category: Jensen JL]]