6cb2: Difference between revisions

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<StructureSection load='6cb2' size='340' side='right'caption='[[6cb2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='6cb2' size='340' side='right'caption='[[6cb2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cb2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CB2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CB2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CB2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">uppP, bacA, upk, b3057, JW3029 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Undecaprenyl-diphosphate_phosphatase Undecaprenyl-diphosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.27 3.6.1.27] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cb2 OCA], [https://pdbe.org/6cb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cb2 RCSB], [https://www.ebi.ac.uk/pdbsum/6cb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cb2 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cb2 OCA], [http://pdbe.org/6cb2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cb2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cb2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cb2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/UPPP_ECOLI UPPP_ECOLI]] Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.<ref>PMID:15778224</ref> 
[https://www.uniprot.org/uniprot/UPPP_ECO57 UPPP_ECO57] Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Undecaprenyl pyrophosphate phosphatase (UppP) is an integral membrane protein that recycles the lipid carrier essential to the ongoing biosynthesis of the bacterial cell wall. Individual building blocks of peptidoglycan are assembled in the cytoplasm on undecaprenyl phosphate (C55-P) before being flipped to the periplasmic face, where they are polymerized and transferred to the existing cell wall sacculus, resulting in the side product undecaprenyl pyrophosphate (C55-PP). Interruption of UppP's regeneration of C55-P from C55-PP leads to the buildup of cell wall intermediates and cell lysis. We present the crystal structure of UppP from Escherichia coli at 2.0 A resolution, which reveals the mechanistic basis for intramembranal phosphatase action and substrate specificity using an inverted topology repeat. In addition, the observation of key structural motifs common to a variety of cross membrane transporters hints at a potential flippase function in the specific relocalization of the C55-P product back to the cytosolic space.
 
Crystal structure of an intramembranal phosphatase central to bacterial cell-wall peptidoglycan biosynthesis and lipid recycling.,Workman SD, Worrall LJ, Strynadka NCJ Nat Commun. 2018 Mar 20;9(1):1159. doi: 10.1038/s41467-018-03547-8. PMID:29559664<ref>PMID:29559664</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6cb2" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Undecaprenyl pyrophosphate synthase|Undecaprenyl pyrophosphate synthase]]
*[[Undecaprenyl pyrophosphate synthase 3D structures|Undecaprenyl pyrophosphate synthase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Undecaprenyl-diphosphate phosphatase]]
[[Category: Strynadka NCJ]]
[[Category: Strynadka, N C.J]]
[[Category: Workman SD]]
[[Category: Workman, S D]]
[[Category: Worrall LJ]]
[[Category: Worrall, L J]]
[[Category: Hydrolase]]
[[Category: Phosphatase]]
[[Category: Pyrophosphate]]
[[Category: Undecaprenyl]]

Latest revision as of 17:27, 13 March 2024

Crystal structure of Escherichia coli UppPCrystal structure of Escherichia coli UppP

Structural highlights

6cb2 is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UPPP_ECO57 Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.

See Also

6cb2, resolution 2.00Å

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