6olt: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 6olt is ON HOLD
+==Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP==
+<StructureSection load='6olt' size='340' side='right'caption='[[6olt]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OLT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRJ:N-[2-(dodecanoylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide'>MRJ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6olt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6olt OCA], [https://pdbe.org/6olt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6olt RCSB], [https://www.ebi.ac.uk/pdbsum/6olt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6olt ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Carbon-carbon bond forming reactions are essential transformations in natural product biosynthesis. During de novo fatty acid and polyketide biosynthesis, beta-ketoacyl-acyl carrier protein (ACP) synthases (KS), catalyze this process via a decarboxylative Claisen-like condensation reaction. KSs must recognize multiple chemically distinct ACPs and choreograph a ping-pong mechanism, often in an iterative fashion. Here, we report crystal structures of substrate mimetic bearing ACPs in complex with the elongating KSs from Escherichia coli, FabF and FabB, in order to better understand the stereochemical features governing substrate discrimination by KSs. Complemented by molecular dynamics (MD) simulations and mutagenesis studies, these structures reveal conformational states accessed during KS catalysis. These data taken together support a gating mechanism that regulates acyl-ACP binding and substrate delivery to the KS active site. Two active site loops undergo large conformational excursions during this dynamic gating mechanism and are likely evolutionarily conserved features in elongating KSs.
-Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P.
+Gating mechanism of elongating beta-ketoacyl-ACP synthases.,Mindrebo JT, Patel A, Kim WE, Davis TD, Chen A, Bartholow TG, La Clair JJ, McCammon JA, Noel JP, Burkart MD Nat Commun. 2020 Apr 7;11(1):1727. doi: 10.1038/s41467-020-15455-x. PMID:32265440<ref>PMID:32265440</ref>
-Description: Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Chen, A]]
+<div class="pdbe-citations 6olt" style="background-color:#fffaf0;"></div>
-[[Category: Bartholow, T.G]]
-[[Category: Noel, J.P]]
+==See Also==
-[[Category: Davis, T.D]]
+*[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]]
-[[Category: Burkart, M.D]]
+*[[Acyl carrier protein synthase 3D structures|Acyl carrier protein synthase 3D structures]]
-[[Category: Mindrebo, J.T]]
+== References ==
-[[Category: Kim, W.E]]
+<references/>
-[[Category: La Clair, J]]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Bartholow TG]]
+[[Category: Burkart MD]]
+[[Category: Chen A]]
+[[Category: Davis TD]]
+[[Category: Kim WE]]
+[[Category: La Clair J]]
+[[Category: Mindrebo JT]]
+[[Category: Noel JP]]