3b42: Difference between revisions

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==Periplasmic sensor domain of chemotaxis protein GSU0935==
The line below this paragraph, containing "STRUCTURE_3b42", creates the "Structure Box" on the page.
<StructureSection load='3b42' size='340' side='right'caption='[[3b42]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3b42]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B42 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
{{STRUCTURE_3b42|  PDB=3b42  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b42 OCA], [https://pdbe.org/3b42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b42 RCSB], [https://www.ebi.ac.uk/pdbsum/3b42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b42 ProSAT]</span></td></tr>
 
</table>
'''Periplasmic sensor domain of chemotaxis protein GSU0935'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q74EM7_GEOSL Q74EM7_GEOSL]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Periplasmic sensor domains from two methyl-accepting chemotaxis proteins from Geobacter sulfurreducens (encoded by genes GSU0935 and GSU0582) were expressed in Escherichia coli. The sensor domains were isolated, purified, characterized in solution, and their crystal structures were determined. In the crystal, both sensor domains form swapped dimers and show a PAS-type fold. The swapped segment consists of two helices of about 45 residues at the N terminus with the hemes located between the two monomers. In the case of the GSU0582 sensor, the dimer contains a crystallographic 2-fold symmetry and the heme is coordinated by an axial His and a water molecule. In the case of the GSU0935 sensor, the crystals contain a non-crystallographic dimer, and surprisingly, the coordination of the heme in each monomer is different; monomer A heme has His-Met ligation and monomer B heme has His-water ligation as found in the GSU0582 sensor. The structures of these sensor domains are the first structures of PAS domains containing covalently bound heme. Optical absorption, electron paramagnetic resonance and NMR spectroscopy have revealed that the heme groups of both sensor domains are high-spin and low-spin in the oxidized and reduced forms, respectively, and that the spin-state interconversion involves a heme axial ligand replacement. Both sensor domains bind NO in their ferric and ferrous forms but bind CO only in the reduced form. The binding of both NO and CO occurs via an axial ligand exchange process, and is fully reversible. The reduction potentials of the sensor domains differ by 95 mV (-156 mV and -251 mV for sensors GSU0582 and GSU0935, respectively). The swapped dimerization of these sensor domains and redox-linked ligand switch might be related to the mechanism of signal transduction by these chemotaxis proteins.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b4/3b42_consurf.spt"</scriptWhenChecked>
3B42 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B42 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Structures and solution properties of two novel periplasmic sensor domains with c-type heme from chemotaxis proteins of Geobacter sulfurreducens: implications for signal transduction., Pokkuluri PR, Pessanha M, Londer YY, Wood SJ, Duke NE, Wilton R, Catarino T, Salgueiro CA, Schiffer M, J Mol Biol. 2008 Apr 11;377(5):1498-517. Epub 2008 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18329666 18329666]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b42 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Geobacter sulfurreducens]]
[[Category: Geobacter sulfurreducens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Pokkuluri, P R.]]
[[Category: Pokkuluri PR]]
[[Category: Schiffer, M]]
[[Category: Schiffer M]]
[[Category: C-type heme containing sensor]]
[[Category: Chemotaxis]]
[[Category: Geobacter sulfurreducen]]
[[Category: Pas domain]]
[[Category: Signaling protein]]
[[Category: Unknown function]]

Latest revision as of 12:27, 21 February 2024

Periplasmic sensor domain of chemotaxis protein GSU0935Periplasmic sensor domain of chemotaxis protein GSU0935

Structural highlights

3b42 is a 2 chain structure with sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q74EM7_GEOSL

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

3b42, resolution 1.90Å

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OCA
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