|
|
| (2 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| <StructureSection load='2avi' size='350' side='right' scene='41/410356/Cv/4' caption='The avidin tetramer complex with biotin (PDB code [[2avi]])'> | | <StructureSection load='2avi' size='350' side='right' scene='41/410356/Cv/4' caption='The avidin tetramer complex with biotin (PDB code [[2avi]])'> |
| | __TOC__ |
| | ==Function== |
|
| |
|
| [[Avidin]] '''(Av)''' is a protein found in egg white which binds biotin (vitamin B7) with high affinity. This property makes it a powerful tool in various protein pull-down assays. Similar proteins are:<br /> | | [[Avidin]] '''(Av)''' is a protein found in egg white which binds biotin (vitamin B7) with high affinity. This property makes it a powerful tool in various protein pull-down assays. Similar proteins are:<br /> |
| Line 10: |
Line 12: |
| * '''Hoefavidin''' from ''Hoeflea phototrophica''<br /> | | * '''Hoefavidin''' from ''Hoeflea phototrophica''<br /> |
| * '''Shwanavidin''' from ''Shewanella dentrifica''<br /> | | * '''Shwanavidin''' from ''Shewanella dentrifica''<br /> |
| | * '''Wilavidin''' from ''Gammaproteobacteria bacterium'' forms biotin-binding hexameters<br /> |
| * '''AVR2''' and '''AVR4''' are avidin-related proteins. | | * '''AVR2''' and '''AVR4''' are avidin-related proteins. |
|
| |
|
| Line 29: |
Line 32: |
| * [[Molecular Playground/Streptavidin]] | | * [[Molecular Playground/Streptavidin]] |
| * [[Streptavidin Binding Site]] | | * [[Streptavidin Binding Site]] |
| * [[Hila Cohen/Test Page]]. | | * [[Hila Cohen/Test Page]] |
| | * [[Molecular Playground/Biotin binding avidin]] |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 38: |
Line 42: |
| | | |
| </StructureSection> | | </StructureSection> |
| == 3D structures of Avidin ==
| |
|
| |
| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| |
|
| |
| *Avidin
| |
|
| |
| **[[3fdc]], [[2jgs]], [[2c4i]], [[1vyo]], [[1rav]], [[1ave]] – cAv – chicken<br />
| |
| **[[2a5b]], [[2a5c]], [[2a8g]] – cAv+deoxyribose<br />
| |
| **[[1nqn]], [[2cam]], [[4bcs]], [[4u46]], [[5lur]] – cAv (mutant)<br />
| |
| **[[1ldo]], [[1ldq]], [[1lel]], [[1ij8]], [[1avd]], [[2avi]], [[3vgw]], [[3vhh]], [[3vhi]], [[3vhm]], [[4i60]], [[4jhq]], [[5hlm]], [[5iru]], [[5irw]], [[5myq]] – cAV + biotin derivative<br />
| |
| **[[5chk]] – cAV+ HABA<br />
| |
| **[[3szh]], [[3szi]] – SdAV – ''Shewanella denitrificans''<br />
| |
| **[[3t2x]] – SdAV (mutant)<br />
| |
| **[[3szj]] – SdAV + biotin<br />
| |
| **[[3t2w]] – SdAV (mutant) + biotin
| |
|
| |
| * Streptavidin
| |
|
| |
| **[[2bc3]], [[2iza]], [[2izb]], [[2izc]], [[2izd]], [[2ize]], [[2rta]], [[2rtb]], [[2rtc]], [[3ry1]], [[4gd9]]. [[4gda]], [[4gjs]] - StrAv - ''Streptomyces avidinii''<br />
| |
| **[[3mm0]], [[3pk2]], [[3rds]], [[3rdx]], [[3re5]], [[3re6]], [[4gjv]] - StrAv (mutant) <br />
| |
| **[[1swa]], [[1swb]], [[1swc]], [[1ry1]] - StrAv core<br />
| |
| **[[1swh]], [[1swj]], [[1swl]], [[1swo]], [[1swq]], [[1sws]], [[2y3e]], [[3t6l]] - StrAv core (mutant)<br />
| |
| **[[4jnj]] – StrAv/rhizavidin<br />
| |
| **[[4jo6]] – StrAv + SBP-TAG<br />
| |
|
| |
| *''Streptavidin binary complexes''
| |
|
| |
| **[[1rxh]] – StrAv (mutant)+ biotinyl p-nitroaniline<br />
| |
| **[[1rxj]], [[1rxk]], [[1nqm]], [[1kff]], [[1kl4]], [[1luq]], [[1mm9]], [[1moy]], [[1n4j]], [[1n7y]], [[1n9y]], [[1nbx]], [[1swf]], [[1swu]], [[1kl3]], [[1kl5]] - StrAv (mutant) + strep-tag II peptide<br />
| |
| **[[1rst]], [[1rsu]] - StrAv + strep-tag II peptide<br />
| |
| **[[1df8]], [[1mep]], [[1n43]], [[1n9m]], [[1ndj]], [[1swg]], [[3rdm]], [[3rdo]], [[4ekv]] - StrAv (mutant)+biotin<br />
| |
| **[[1nc9]], [[3rdq]], [[4dne]] - StrAv (mutant)+biotin derivative<br />
| |
| **[[2izl]], [[2rtl]], [[2rtm]], [[2rtn]], [[2rto]], [[2rtp]], [[2rtq]], [[2rtr]] - StrAv +iminobiotin<br />
| |
| **[[1mk5]], [[1swd]], [[1swe]], [[2f01]], [[2gh7]], [[3ry2]] - StrAv core+biotin<br />
| |
| **[[1swk]], [[1swn]], [[1swp]], [[1swr]], [[1swt]], [[3mg5]], [[2y3f]], [[3t6f]] - StrAv core (mutant) + biotin<br />
| |
| **[[1stp]], [[2izf]], [[2izg]], [[2izh]], [[2izi]], [[2izj]], [[2rtd]], [[2rte]], [[2rtf]], [[2rtg]] - StrAv +biotin<br />
| |
| **[[3rdu]] - StrAv (mutant) + PEG<br />
| |
| **[[1hqq]], [[1hxl]] – StrAv + MP-2 (mutant)<br />
| |
| **[[1hy2]] - StrAv + MP-1 <br />
| |
| **[[1hxz]] - StrAv + MP-2<br />
| |
| **[[1pts]], [[1sld]], [[1sle]], [[1slg]], [[1str]], [[1sts]], [[1vwa]], [[1vwb]], [[1vwc]], [[1vwd]], [[1vwe]], [[1vwf]], [[1vwg]], [[1vwh]], [[1vwi]], [[1vwj]], [[1vwk]], [[1vwl]], [[1vwm]], [[1vwn]], [[1vwo]], [[1vwp]], [[1vwq]], [[1vwr]], [[2g5l]] - StrAv + peptide<br />
| |
| **[[1slf]] - StrAv + SO4<br />
| |
| **[[1sre]], [[1srf]], [[1srg]], [[1srh]], [[1sri]], [[1srj]] - StrAv + benzoic acid ligands<br />
| |
| **[[1lcv]], [[1lcw]], [[1lcz]], [[1i9h]] – StrAv+biotin derivative<br />
| |
| **[[2izk]], [[2rth]], [[2rti]], [[2rtj]], [[2rtk]] – StrAV+glycouril<br />
| |
| **[[2qcb]], [[2wpu]] – StrAv+Ru ligand
| |
|
| |
| * Rhizavidin
| |
|
| |
| **[[3ew1]] – RhiAv – ''Rhizobium etli''<br />
| |
| **[[3ew2]] – RhiAV+biotin<br />
| |
|
| |
| * Xenavidin
| |
|
| |
| **[[2uz2]], [[2uyw]] – XenAv – frog<br />
| |
|
| |
| * Tamavidin
| |
|
| |
| **[[2zsc]] – TanAv – Tamogitake mushroom
| |
|
| |
| * Bradavidin
| |
|
| |
| **[[4ggr]], [[4ggt]] – BjAv – ''Bradyrhizobium japonicum''<br />
| |
| **[[4ggz]] – BjAv + biotin<br />
| |
|
| |
| *Hoefavidin
| |
|
| |
| **[[4z6j]] – HpAv – ''Hoeflea phototrophica''<br />
| |
| **[[4z27]] – HpAv short <br />
| |
| **[[4z28]] – HpAv short + biotin <br />
| |
| **[[4z2p]], [[4z2v]], [[4z2o]] – HpAv short + peptide <br />
| |
|
| |
| *Shwanavidin
| |
|
| |
| **[[3szi]], [[3szh]] – SdAv – ''Shewanella dentrifica''<br />
| |
| **[[3t2x]] – SdAv (mutant)<br />
| |
| **[[3szj]] – SdAv + biotin<br />
| |
| **[[3t2w]] – SdAv (mutant) + biotin<br />
| |
|
| |
| * Zebavidin
| |
|
| |
| **[[4bj8]] – Av – zebrafish<br />
| |
|
| |
| * Avidin-related protein AVR2
| |
|
| |
| **[[1wbi]] – cAVR2<br />
| |
|
| |
| *Avidin-related protein AVR4
| |
|
| |
| **[[2fhl]] – cAVR4+biotinyl p-nitroaniline<br />
| |
| **[[2of9]], [[2ofa]], [[1y53]], [[3mm0]] - cAVR4 (mutant)<br />
| |
| **[[2fhn]], [[2of8]], [[2ofb]] - cAVR4 (mutant)+biotinyl p-nitroaniline<br />
| |
| **[[1y52]], [[1y55]] - cAVR4 (mutant)+biotin<br />
| |
| **[[2mf6]] - cAVR4/Av (mutant)+biotin – NMR<br />
| |
| }}
| |
|
| |
|
| ==Reference== | | ==Reference== |