Exoenzyme: Difference between revisions

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<StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'>
<StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'>


'''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells.  The breakdown components can pass through the cell wall.  '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> .
'''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells.  The breakdown components can pass through the cell wall.   
*'''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate.  
* '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> .


<scene name='59/593306/Cv/6'>NAD binding site</scene>.
<scene name='59/593306/Cv/6'>NAD binding site</scene>.


<scene name='59/593306/Cv/7'>ADP binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]). <ref>PMID:18369192</ref>  
<scene name='59/593306/Cv/7'>ADP binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]). <ref>PMID:18369192</ref>  
</StructureSection>


==3D structures of exoenzyme==
==3D structures of exoenzyme==
[[Exoenzyme 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Exoenzyme C3
 
**[[1g24]] – CbExo residues 8-218 – ''Clostridium butulinum''<br />
**[[1uzi]], [[1r45]], [[1r4b]], [[2c89]] – CbExo catalytic domain <br />
**[[1gze]], [[2c8b]], [[2c8d]], [[2c8e]], [[2c8g]] – CbExo catalytic domain (mutant)<br />
**[[3bw8]] - Exo catalytic domain - ''Clostridium limosum''<br />
 
*Exoenzyme C3 complexes
 
**[[1gzf]] – CbExo catalytic domain + pyridinium derivative + ADP + NAD<br />
**[[2bov]] – CbExo + GDP + Ral-A GTPase<br />
**[[2a78]] – CbExo catalytic domain + GDP + Ral-A GTPase<br />
**[[2a9k]] – CbExo catalytic domain+ GDP + Ral-A GTPase + NAD<br />
**[[2c8a]] – CbExo catalytic domain + nicotinamide<br />
**[[2c8c]] – CbExo catalytic domain (mutant) + ADP + NAD<br />
**[[2c8f]], [[2c8h]] – CbExo catalytic domain (mutant) + NAD<br />
 
*Exoenzyme S
 
**[[1r4t]] – PaExo S GAP domain – ''Pseudomonas aeruginosa'' - NMR<br />
**[[1he1]] – PaExo S GAP domain + Ras-like protein + GDP <br />
**[[1he9]] – PaExo S GAP domain + Ras-like protein <br />
**[[4zua]] – PaExo SA regulatory domain residues 2-178 <br />
**[[3kxy]] – PaExo SC residues 1-133 + PaExo SE residues 16-81<br />
 
*Exoenzyme T
 
**[[4jmf]] – PaExo residues 28-77 + chaperone<br />
}}


== References ==
== References ==

Latest revision as of 12:32, 20 June 2024


Exoenzymes (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall.

  • Exo C3 is a Clostridium butulinum toxin which binds NAD, splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate.
  • Exo S and Exo T are secreted by Pseudomonas aeruginosa and has GTPase-activating activity as well as ADP-ribosyltransferase activity[1] .

.

in exoenzyme C3 catalytic domain (PDB code 2c8c). [2]

3D structures of exoenzyme

Exoenzyme 3D structures


Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code 2c8c).

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Han S, Arvai AS, Clancy SB, Tainer JA. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J Mol Biol. 2001 Jan 5;305(1):95-107. PMID:11114250 doi:10.1006/jmbi.2000.4292
  2. ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508

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Michal Harel, Alexander Berchansky
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