6nu3: Difference between revisions
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New page: '''Unreleased structure''' The entry 6nu3 is ON HOLD Authors: Sharma, M.R., Koripella, R.K., Agrawal, R.K. Description: Structural insights into unique features of the human mitochondr... |
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The | ==Structural insights into unique features of the human mitochondrial ribosome recycling== | ||
<SX load='6nu3' size='340' side='right' viewer='molstar' caption='[[6nu3]], [[Resolution|resolution]] 4.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6nu3]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NU3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nu3 OCA], [https://pdbe.org/6nu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nu3 RCSB], [https://www.ebi.ac.uk/pdbsum/6nu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nu3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RM45_HUMAN RM45_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for synthesizing proteins that are essential for oxidative phosphorylation (ATP generation). Despite their common ancestry with bacteria, the composition and structure of the human mitoribosome and its translational factors are significantly different from those of their bacterial counterparts. The mammalian mitoribosome recycling factor (RRFmt) carries a mito-specific N terminus extension (NTE), which is necessary for the function of RRFmt Here we present a 3.9-A resolution cryo-electron microscopic (cryo-EM) structure of the human 55S mitoribosome-RRFmt complex, which reveals alpha-helix and loop structures for the NTE that makes multiple mito-specific interactions with functionally critical regions of the mitoribosome. These include ribosomal RNA segments that constitute the peptidyl transferase center (PTC) and those that connect PTC with the GTPase-associated center and with mitoribosomal proteins L16 and L27. Our structure reveals the presence of a tRNA in the pe/E position and a rotation of the small mitoribosomal subunit on RRFmt binding. In addition, we observe an interaction between the pe/E tRNA and a mito-specific protein, mL64. These findings help understand the unique features of mitoribosome recycling. | |||
Structural insights into unique features of the human mitochondrial ribosome recycling.,Koripella RK, Sharma MR, Risteff P, Keshavan P, Agrawal RK Proc Natl Acad Sci U S A. 2019 Apr 8. pii: 1815675116. doi:, 10.1073/pnas.1815675116. PMID:30962385<ref>PMID:30962385</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Agrawal | <div class="pdbe-citations 6nu3" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
*[[Ribosome recycling factor|Ribosome recycling factor]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Agrawal RK]] | |||
[[Category: Koripella RK]] | |||
[[Category: Sharma MR]] | |||
Latest revision as of 13:16, 23 October 2024
Structural insights into unique features of the human mitochondrial ribosome recyclingStructural insights into unique features of the human mitochondrial ribosome recycling
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